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Example sentences for: sbcbc
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In the recBC sbcBC strain, RecBCD enzyme is inactive and RecFOR and RecQJ proteins promote recombination together with RecA [ 19 ] . In the recBC sbcBC background, recA, recF, recJ, recQ and recR mutants accumulated these huge linears to varying extents (Figure 2D).
The recE gene product of the Rac prophage converts dsDNA ends into 3' protruding single-stranded form and the recT gene product promotes recombination by annealing them with a homologous DNA in its vicinity (for review, see [ 7 8 ] ). This recombination may result in one progeny DNA (non-conservative recombination) or two progeny DNAs (conservative double-strand break repair) [ 9 ] . In a recBC sbcBC background, a ds end stimulates homologous recombination that results in only one progeny DNA (non-conservative recombination) [ 10 ] . Analysis of the stimulation of recombination by replication (for review, see [ 11 ] ) and analysis of altered chromosomal replication (for review, see [ 12 ] ) led to the proposal that a chromosomal ds break formed during replication fork arrest triggers homologous recombination, which would reconstitute a replication fork (for review, see [ 5 ] ).
In the recBC sbcA and the recBC sbcBC backgrounds, the absence of RecBCD nuclease may prevent faster degradation of the large non-circular DNAs.
In this work, we employed the pulsed-field gel electrophoresis procedure to measure large non-circular forms of the chromosome obtained from various recombination-defective mutants in rec +, recBC sbcA , and recBC sbcBC genetic backgrounds.
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