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Example sentences for: sbca

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In this work, we employed the pulsed-field gel electrophoresis procedure to measure large non-circular forms of the chromosome obtained from various recombination-defective mutants in rec +, recBC sbcA , and recBC sbcBC genetic backgrounds.
In the recBC sbcA and the recBC sbcBC backgrounds, the absence of RecBCD nuclease may prevent faster degradation of the large non-circular DNAs.
The accumulation of the non-circular, broken chromosomes correlated with the growth rate or DNA damage response in most of the recBC -minus background [ 30 ] . The recB or recC null mutation showed low viability even in the absence of exogenous DNA damage [ 31 32 ] . A simple interpretation of these data is that RecA, RecFOR, and RecQJ functions (and RecET functions for the sbcA background) repair chromosome breakage and/or prevent generation of the breakage.
In the recBC sbcA strain, an sbcA mutation on the Rac prophage expresses recET genes, which promotes homologous recombination at a ds end [ 7 ] . The accumulation of the huge linears was seen with recA, recE, recT, recJ, recQ, recF, recO and recR strains (Figure 2C).
The accumulation by the recJ mutation in the recBC sbcA background is suppressed by a recQ mutation (Figure 2C).