Words similar to sali
Example sentences for: sali
How can you use “sali” in a sentence? Here are some example sentences to help you improve your vocabulary:
Gal-GRIP1 NR box (1,2,3) fusion (amino acids 610-770) was prepared by PCR amplification of the appropriate region of GRIP1 (primers obtained from Biomolecular Resource Center, UCSF) containing EcoRI and SalI sites, the PCR fragment was digested with these enzymes and subcloned into the pM GAL4 expression vector (Clontech Laboratories, Inc.
The resulting plasmid was called pBigT, and its MCS contains sites for the restriction enzymes NheI, SalI, AccI, XhoI, ApaI, SacII, NotI, SacI and BclI.
There are a number of important features associated with this vector that are illustrated in Fig 2. This plasmid includes a unique SalI restriction endonuclease cut-site for insertion of open reading frames, followed by intron and poly- [A] addition sequences for correct post-transcriptional processing of transgenic RNA.
This was inserted downstream of the SA in pPacSA digested with SalI, Klenow filled, and then cut with KpnI.
The PCR product was cloned into the NotI and SalI sites in PMI-hCD2 [ 46 ] . The coding sequence for the Rfz-2/β 2 AR chimeric receptor in CS2+ [ 6 37 ] was digested with EcoRI and XhoI and cloned into PMI-hCD2 at the EcoRI and SalI sites.
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