Example sentences for: sal

How can you use “sal” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Trees/shrubs: bahera, bel, bendy, caraunda, dhak, dhaman, dhauri, dhawa, haldu, jaman, jambo, jambos, jambool, kapur, karela, kathal, kikar, kokan, neem, palas, pipal, sal, salai, sunn, susco, toon

  • The 1.2 kb coding sequence of the receptor was subcloned into pCI-neo ®mammalian expression vector (Promega, Madison, WI) using the restriction sites EcoRI and Sal I. HEK 293 cell were transfected with the vector using the lipofectin ®reagent (Life Technologies, Rockville, MD).

  • Yesterday, Deputy Mayor Sal Sirabella said he had not seen the lawsuit.

  • This cloning strategy left a unique Sal I site into which coding sequences of transgenes could be introduced.

  • A fragment containing the PPT1 ORF was excised from the pBII-PPT1 using Eco RI and Xho I restriction enzymes and was subcloned downstream of the GST coding region into the Eco RI and Sal I sites of the bacterial expression vector pET-21a GST [ 37 ] . To construct the pET-GST-PPT1 (1-504) plasmid a 453 bp fragment was amplified from pET-GST-PPT1 by PCR using a 3' primer containing a Xho I restriction site and a stop codon designed to truncate the PPT1 gene product at Met504 (5'-gatcctcgagTTAcattggttttatatctggg) and a 5' primer (5'-gtaatgcatggtggttta) encompassing the Nsi I restriction site of PPT1 . The PCR product was cloned into the Nsi I and Xho I restriction sites of pET-GST-PPT1 and sequenced.


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