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Example sentences for: s-
How can you use “s-” in a sentence? Here are some example sentences to help you improve your vocabulary:
Mounted specimens were then sputter coated with gold and viewed on a Hitachi S-3500N scanning electron microscope (Tokyo, Japan) at 1-5 kV.
After denaturation in 6 M and renaturation in 0.187 M guanidine-HCl in HB buffer (25 mM Hepes pH 7.2, 5 mM NaCl, 5 mM MgCl2, 1 mM DTT), the blots were saturated at 4°C in buffer H (20 mM Hepes pH 7.7, 7.75 mM KCl, 0.1 mM EDTA, 25 mM MgCl 2 , 1 mM DTT, 0.05% NP40, 1% dry milk) for 2 hours, and then incubated for 2 hours at 4°C with 50 μl of 35S-methionine-labeled in vitro-translated hCCR4 or luciferase protein (synthesized using a reticulocyte lysate-coupled transcription/translation kit [Promega]).
Under conditions favoring proliferation, the phosphorylation of pRB via cyclin-dependent kinases (CDKs) in mid-to-late G 1 phase results in liberation of E2F and other pRB-bound transcription factors, which then activate the transcription of S-phase genes [ 19 ] . In late M phase, pRB returns to its growth suppressive, hypophosphorylated form due to the action of a pRB-directed protein phosphatase belonging to the type 1 class of serine/threonine protein phosphatases [ 11 20 21 ] .
An increase of oxidative stress in RPE is associated with an increase of cellular catalase, metallothionein [ 3 ] and glutathione S-transferase [ 35 ] , which should serve as a protective mechanism to decrease the cytotoxicity caused by H 2 O 2 and other reactive oxygen species.
Naturally occurring analogs of purine and pyrimidine bases have been examined extensively for their ability to increase the thermodynamic stability of DNA:DNA and DNA:RNA duplexes [ 11 12 13 14 15 ] . Among these are 2-aminoadenine also known as diaminopurine (DAP), which is found in S-2L cyanophage DNA [ 16 ] , 5-methyl uracil (MeU), and 5-methyl cytidine (MeC).
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