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Example sentences for: rpmh
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The sequence immediately flanking the dnaA gene is considered the origin of chromosomal replication, or oriC region [ 9 10 ] . This region contains several genes that encode proteins required for basic cellular functions, including the protein subunit of RNase P (RnpA), ribosomal protein L34 (RpmH), the replication initiator protein (DnaA), the beta subunit of DNA polymerase III (DnaN), the recombination repair protein RecF, and the DNA gyrase proteins GyrA and GyrB.
unknown, but it may have a role in cell division [ 11 ] . RNase P, which consists of the protein subunit RnpA and a catalytic RNA subunit, is essential for generating mature tRNAs by cleaving the 5'-terminal leader sequences of precursor tRNAs [ 16 ] . rpmH encodes ribosomal protein L34, and DnaA is the initiator protein for chromosome replication.
The M. smegmatis coding sequence, gnd , has similarity to the 6-phophogluconate dehydrogenase genes in E. coli , but the mycobacterial protein is predicted to be about 200 amino acids shorter than the E. coli homolog . The length of non-coding intergenic regions between rpmH - dnaA and dnaA - dnaN is well conserved among the bacteria shown in figure 2. In many bacteria where a functional oriC has been identified, this gene order is conserved and oriC is adjacent to the dnaA gene [ 9 10 19 ] .
For instance, there was complete nucleotide identity in the rpmH and recF genes and only 94% identity in the gene rnpA . To verify that these observed differences were real and not as a result of sequencing errors in the yet unfinished M. avium genome, we confirmed the data by resequencing the entire 11 kb region from an isolate clone of M. avium and obtained identical results (not shown).
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