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Example sentences for: rockville

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At the beginning of each experiment, cells were washed three times with Hank's buffered sterile saline (Cellgro) to remove traces of serum and were placed in Dulbecco's modified Eagle's medium containing 0.2% lactalbumin hydrolysate (Gibco/BRL, Rockville, MD, USA).
The human embryonic cell line HEL 299 (American Type Culture Collection, Rockville, MD) was used for P. carinii culture.
Lens halves were dehydrated in an ascending ethanol series from 50-100 %. Once lens halves were in 100% ethanol, they were critical point dried in carbon dioxide in a Samdri-790B (Tousimis Research Corp., Rockville, MD).
Mononucleated cells were resuspended in plating medium containing 78% Dulbecco's modified Eagle's medium (DMEM) with low glucose (Life Technologies, Rockville, MD), 10 % horse serum (HS, Sigma, St. Louis, MO), 10 % fetal bovine serum (FBS, Sigma, St. Louis, MO), 2% chicken embryo extract (CEE, Life Technologies, Rockville, MD) and plated on plastic culture dishes coated with gelatin at a density of ∼ 1 × 10 4cells per dish.
The bacteria were sedimented by centrifugation and resuspended in 3 mL Trizol (Gibco, Rockville, MD).