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Example sentences for: rockville

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RNA was then used for the first-strand cDNA synthesis in the SuperScript Preamplification System (GIBCO, BRL, Rockville, MD, USA) in accordance with the manufacturer's instructions.
Human SW-1353 chondrosarcoma cells were purchased from the American Type Culture Collection (Rockville, MD, USA).
Polyclonal goat anti-murine MIG/CXCL9 and anti-murine IP-10/CXCL10 specific anti-serum were produced and characterized as previously described [ 31 ] . Anti-mouse IFNγ monoclonal (R4-462, American Type Culture Collection, Rockville, Maryland, MD) neutralizing antibody was purified by affinity chromatography from SCID mice ascites, which was generated 3-4 weeks after intraperitoneal injection of 10 6R4-462 hybridoma cells per mouse [ 32 ] . For flow cytometry, labeled antibodies (FITC, PE, PerCP) against the T cell surface markers to CD3, CD4, CD8 and against the DC markers, CD11c and DEC 205 and the appropriate controls were purchased from PharMingen.
RPMI 1640 media, fetal bovine serum, and GlutaMAX™-I were obtained from Life Technologies (Rockville, MD).
Human Jurkat and HEp-2 cells were obtained from American Type Culture Collection (Rockville, MD, USA) and were cultured in RPMI 1640 and Dulbecco's modified Eagle's medium (Life Technologies, Rockville, MD, USA), respectively, containing 10% fetal bovine serum under standard conditions.