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Example sentences for: rockland

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The amplified 169-base-pair fragment was digested with 0.4 U of the restriction enzyme Mnl I (New England Bio Labs, Beverly, MA) at 37°C for 6-12 hours and the resulting fragments were subjected to electrophoresis on 4% Nu-Sieve GTG agarose gels (FMC Bioproducts, Rockland, ME) and the nucleotide bands visualized by ethidium bromide fluorescence and photography.
Agarose plugs containing bacterial cells were prepared in a 1 ml syringe by combining cells with an equal volume of 2% SeaKem Gold agarose (FMC BioProducts, Rockland, ME) melted in water.
Plates were blocked with 1% BSA (Rockland, Gilbertsville, PA)/PBS at room temperature for 30 minutes.
For NF-κB p65 blotting, rabbit anti-p65 polyclonal (Rockland, Germany) and goat anti-rabbit horseradish peroxidase conjugated antibody (Sigma) were used as primary and secondary antibody respectively at a 1:1000 dilution.
All amplifications were confirmed by gel electrophoresis (3% Metaphor agarose [Cat # 50181, BioWhittaker Molecular Applications, Rockland, ME] in 0.5X TBE [ 25 ] ). Ethidium bromide-stained products that produced obvious, solitary bands after electrophoresis were processed for size analysis.