Example sentences for: rlk-
How can you use “rlk-” in a sentence? Here are some example sentences to help you improve your vocabulary:
We co-transfected Fyn and Rlk-GFP expressing constructs into HeLa cells, and visualized Fyn with anti-Fyn serum and Texas-red conjugated secondary antibody (Fig.
As previously demonstrated, co-expression of kinase-active Fyn and Rlk-GFP in 293T cells led to a marked increase in the tyrosine phosphorylation and kinase activity of wild type Rlk-GFP (Fig.
Point mutations were introduced into Rlk-GFP by a PCR based strategy [ 53] with oligonucleotides encoding the required mutations (Mutated residues are in bold): Y420F (GGAC GAT GAA T TC ATC AGT TCT TCT G), Y91F (GTC AAG GCT CTT T T T GAC TTC CTG CC).
in vitro kinase activity of Rlk-Y91F was also lower (about half that of wild type); however, the kinase activity was still augmented by the presence of Fyn (Fig.
However, phosphorylation of the Y420F mutant was markedly reduced relative to WT Rlk-GFP.
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