Words similar to rjd

Example sentences for: rjd

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Standard methods were employed for the culturing and manipulation of yeast [ 56 ] . All plasmid constructions were based on the pRS vector series [ 57 ] . To replace CDC15 with TRP1, polymerase chain reaction (PCR) products containing TRP1 flanked by 200-base pair (bp) homology to the 5' and 3' untranslated regions of the targeted gene were used to transform diploid strain RJD381.
For the experiment described in Figure 1, cdc15-2 (RJD 619) cells were grown to exponential phase in YPD at 25°C, and shifted to 37°C for three hours so that >95% cells were arrested as large-budded cells.
WS, RA, and RJD contributed to Figures 1- Figure 6B,Figures 6C-6E, and Figure 7, respectively.
To construct [ pMET3-CDC15, URA3 ] (pWS100) and [ pMET3-cdc15-2, URA3 ] (pWS109), the corresponding genes (from start codon to 300 bps downstream of the stop codon) were amplified by PCR from genomic DNA of wild type (RJD381) or cdc15-2 (RJD619) cells, and cloned into the SpeI and SacII sites of RDB620 (a derivative of pRS316 containing the MET3 promoter inserted between the HindIII and EcoRI sites).