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Example sentences for: rjd

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For the experiment described in Figure 1, cdc15-2 (RJD 619) cells were grown to exponential phase in YPD at 25°C, and shifted to 37°C for three hours so that >95% cells were arrested as large-budded cells.
WS, RA, and RJD contributed to Figures 1- Figure 6B,Figures 6C-6E, and Figure 7, respectively.
To construct [ pMET3-CDC15, URA3 ] (pWS100) and [ pMET3-cdc15-2, URA3 ] (pWS109), the corresponding genes (from start codon to 300 bps downstream of the stop codon) were amplified by PCR from genomic DNA of wild type (RJD381) or cdc15-2 (RJD619) cells, and cloned into the SpeI and SacII sites of RDB620 (a derivative of pRS316 containing the MET3 promoter inserted between the HindIII and EcoRI sites).
Standard methods were employed for the culturing and manipulation of yeast [ 56 ] . All plasmid constructions were based on the pRS vector series [ 57 ] . To replace CDC15 with TRP1, polymerase chain reaction (PCR) products containing TRP1 flanked by 200-base pair (bp) homology to the 5' and 3' untranslated regions of the targeted gene were used to transform diploid strain RJD381.