Example sentences for: riα
How can you use “riα” in a sentence? Here are some example sentences to help you improve your vocabulary:
Because the antibodies to D-AKAP1 and RIα were produced in rabbit and mouse, respectively, double-labeling confocal experiments could be performed with these antibodies on the same tissue slice in order to test for colocalization.
Our interest in PKA and PKC activity in the NMJ derives from: (1) a report that RIα not only is enriched there, but also appears to be tethered to the postsynaptic membrane [ 19 ] , (2) the rich literature describing selective PKC isoform activity and functional compartmentalization in the NMJ and skeletal muscle, (3) our discoveries that D-AKAP1 and D-AKAP2 bind to and appear to colocalize with RIα, and often mitochondria [ 15 16 28 ] ; and (4) our finding that gravin binds to both PKA and PKC [ 13 ] .
Importantly, RIα was observed to be concentrated in other vicinities of the presynapse as well.
This putative association is strengthened by finding for the first time that targeting of RIα requires the AKAP binding surface [ 17 ] . Yotiao is another AKAP enriched at the NMJ, but appears to bind only RII [ 30 ] . PKA phosphorylates the nAChR when stimulated at the synapse by the neuropeptide CGRP and by adenosine from muscle responding to acetylcholine [reviewed by [ 31 ] ]. This phosphorylation may regulate the degradation of the nAChR [ 32 ] . Consistent with the action at the presynapse, it was shown that PKA facilitation of acetylcholine release is dependent on nerve endplate calcium concentration [ 33 ] . Based on our observation of colocalization with synaptophysin at presynaptic vesicles, RIα may be the isotype that influences this release of neurotransmitter.
As with RIα, labeling of the actin region by anti-D-AKAP1 was dominant (fig.
Loading...