Example sentences for: riα
How can you use “riα” in a sentence? Here are some example sentences to help you improve your vocabulary:
Rabbit anti-PKA RIα was prepared by synthesizing the N-terminal 18 residues of human RIα protein and conjugating this peptide (MESGSTAASEEARSLREC) to keyhole limpet haemocyanin (KLH) via the bifunctional reagent, m-maleimidobenzoyl-N-hydroxysuccinimide ester (MES).
Because the labeling patterns for RIα and D-AKAP1 appear similar in the muscle fibers and at the postsynaptic membrane, D-AKAP1 may anchor RIα in these regions, which further supports the cloning and biochemical evidence of interaction between these proteins [ 15 28 ] . Phalloidin was used as a positive control for the actin region of muscle fibers (fig.
Our interest in PKA and PKC activity in the NMJ derives from: (1) a report that RIα not only is enriched there, but also appears to be tethered to the postsynaptic membrane [ 19 ] , (2) the rich literature describing selective PKC isoform activity and functional compartmentalization in the NMJ and skeletal muscle, (3) our discoveries that D-AKAP1 and D-AKAP2 bind to and appear to colocalize with RIα, and often mitochondria [ 15 16 28 ] ; and (4) our finding that gravin binds to both PKA and PKC [ 13 ] .
Mouse anti-PKA RIα, mouse anti-PKA RIIβ, and mouse anti-PKA RIIα were purchased from Transduction Laboratories.
As with RIα, labeling of the actin region by anti-D-AKAP1 was dominant (fig.