Words similar to rhod-
Example sentences for: rhod-
How can you use “rhod-” in a sentence? Here are some example sentences to help you improve your vocabulary:
The time to achieve a stable state (particularly with respect to effluent PO 2 concentrations) or load rhod-2, and then acquire baseline data and perform 1 intervention is 60-90 minutes, beyond which the preparation is not stable and so more than 1 intervention per mouse heart was not undertaken.
To account for changes in dye concentration, formula 2 needs to be modified to account for changes in absorbance (A rhod-2 , formula 1) due to dye leakage.
Isolated perfused mouse heart measurements of intracellular calcium and energetics have been performed using other methods by other investigators [ 17 18 ] . The unique aspects of the present study are that we use the calcium sensitive fluorescent dye rhod-2 to measure intracellular calcium [ 6 7 ] , and that we have related these measurements to both myocardial oxygen consumption and developed pressure.
where K d is the dissociation constant for rhod-2 and calcium (determined by in-vitro calibration with rhod-2 and myoglobin by del Nido et al, ref [ 20 ] , and confirmed by in-vivo manganese quenching, ref [ 29 ] ) and is 710 nM, F t = fluorescence at time t, F max =maximal fluorescence from tetanized heart, and the fluorescence from the heart assuming rhod-2 had no calcium bound is given by F 0 = F b + a (F max - F b ), where F b is the background counts from the heart prior to dye loading, and a = rhod-2 fluorescence in the absence of calcium / rhod-2 fluorescence in the presence of saturating calcium.
To study this we directly measured developed pressures, myocardial oxygen consumption and used a newly developed technique to measure intracellular calcium in perfused mouse hearts with the calcium sensitive fluorescent dye rhod-2 [ 6 7 ] during calcium-induced inotropy and with dobutamine.