Words similar to rhod-

Example sentences for: rhod-

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The methods used to measure intracellular calcium with rhod-2 in perfused hearts have been previously extensively described [ 6 7 20 ] . Rhod-2 (Molecular Probes, OR, 100 μg) was dissolved with dimethylsulfoxide (DMSO, 4 μL) and dH 2 O (200 μL), and loaded through the coronary perfusate.
For rhod-2 the value of a is approximately 0, thus for rhod-2, F 0 was assumed to be equal to F b .
To account for changes in dye concentration, formula 2 needs to be modified to account for changes in absorbance (A rhod-2 , formula 1) due to dye leakage.
However, one advantage of using rhod-2 is that its long excitation and emission wavelengths relative to other calcium sensitive dyes results in minimal filtering of excitation and emission light and autofluorescence [ 7 20 ] . At these visible range wavelengths light may penetrate between 0.3 and 0.4 mm into the myocardium [ 21 ] which may be significantly deeper than shorter wavelength dyes.
Isolated perfused mouse heart measurements of intracellular calcium and energetics have been performed using other methods by other investigators [ 17 18 ] . The unique aspects of the present study are that we use the calcium sensitive fluorescent dye rhod-2 to measure intracellular calcium [ 6 7 ] , and that we have related these measurements to both myocardial oxygen consumption and developed pressure.