Example sentences for: resuspension

How can you use “resuspension” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • During cell resuspension, 1 ml ice-cold 70% Ethanol was added, in which cells were either stored at -20°C for up to three days, or processed after 20 minute fixation by repelleting.

  • To extract RNA from tissue, 100 mg of rat lung was ground in 6 ml of ice-cold resuspension buffer.

  • Subcellular fractionation of RNA into cytoskeleton-associated and cytosolic fractions was performed as previously described [ 15 34 ] . In brief, HL-60 cells were harvested by centrifugation and resuspension in buffer A (10 mM Tris pH 7.6, 0.25 M sucrose, 25 mM KCl, 5 mM MgCl 2 ) with 0.1% Igepal nonionic detergent (Sigma).

  • Similar results were obtained when PBS was used for resuspension of virus particles instead of lysis buffer.

  • HEL cells were dislodged from the flask with a trypsin-EDTA solution, washed with DEPC-treated PBS, and then resuspended in 6 ml of ice-cold resuspension buffer [10 mM Tris-HCl (pH 7.5), 10 mM KC1, 5 mM MgCl 2 ]. Six ml of hot (70°C) lysis buffer [20 mM Tris-HCl (pH 7.4), 0.4 M NaCI, 40 mM EDTA, 1% β-mercaptoethanol, 1% SDS] and 1 ml of citrate-buffered phenol (pH 4.0) were added to each cell suspension.


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