Words similar to resuspending

Example sentences for: resuspending

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Bacterial cultures were prepared by resuspending the cells from overnight cultures in 10 mM MgCl 2 to the required optical density (OD 600 = 0.05; 1 OD 600 = 10 9colony-forming units (CFU)/ml).
The pellets were washed once with nuclear buffer, and the nuclear extracts were prepared by resuspending the pellets in 0.3 ml buffer containing 20 mM HEPES (pH 7.8), 25% glycerol, 0.42 M NaCl, 1.5 mM MgCl
Listeria were prepared by removing 1 ml of the overnight culture, pelleting by a 1 min Eppendorf centrifuge spin, washing the pellet twice with antibiotic-free media, and resuspending in 1 ml of media.
Whole-cell lysis was performed by resuspending the pellet in 2 × SDS sample buffer, vortexing vigorously to shear the chromosomal DNA, and heating to 70°C for about 5 min.
Cells were harvested, washed four times with 40 ml of ice-cold transport buffer (145 mM NaCl, 1 mM MgCl 2 , 1 mM CaCl 2 , 10 mM glucose, 10 mM HEPES, pH 7.4), titered, and preincubated for 30 min. at 37°C after resuspending 3 × 10 7cells in 1 ml of transport buffer.