Words similar to resuspending
Example sentences for: resuspending
How can you use “resuspending” in a sentence? Here are some example sentences to help you improve your vocabulary:
The nuclear proteins were extracted by resuspending the nuclear pellet with buffer C (50 mM Hepes pH7.
The pellets were washed once with nuclear buffer, and the nuclear extracts were prepared by resuspending the pellets in 0.3 ml buffer containing 20 mM HEPES (pH 7.8), 25% glycerol, 0.42 M NaCl, 1.5 mM MgCl
Bacterial cultures were prepared by resuspending the cells from overnight cultures in 10 mM MgCl 2 to the required optical density (OD 600 = 0.05; 1 OD 600 = 10 9colony-forming units (CFU)/ml).
Whole-cell lysis was performed by resuspending the pellet in 2 × SDS sample buffer, vortexing vigorously to shear the chromosomal DNA, and heating to 70°C for about 5 min.
Total protein levels in the SDS extracts were determined by BCA assay (Pierce) after removing the reducing reagent from SDS buffer by precipitating protein from the extract with 10% trichloroacetic acid, washing in 10% trichloroacetic acid and resuspending the pellet in 0.1 N NaOH.