Words similar to resuspending

Example sentences for: resuspending

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Whole-cell lysis was performed by resuspending the pellet in 2 × SDS sample buffer, vortexing vigorously to shear the chromosomal DNA, and heating to 70°C for about 5 min.
Total protein levels in the SDS extracts were determined by BCA assay (Pierce) after removing the reducing reagent from SDS buffer by precipitating protein from the extract with 10% trichloroacetic acid, washing in 10% trichloroacetic acid and resuspending the pellet in 0.1 N NaOH.
Cells were harvested, washed four times with 40 ml of ice-cold transport buffer (145 mM NaCl, 1 mM MgCl 2 , 1 mM CaCl 2 , 10 mM glucose, 10 mM HEPES, pH 7.4), titered, and preincubated for 30 min. at 37°C after resuspending 3 × 10 7cells in 1 ml of transport buffer.
The pellets were washed once with nuclear buffer, and the nuclear extracts were prepared by resuspending the pellets in 0.3 ml buffer containing 20 mM HEPES (pH 7.8), 25% glycerol, 0.42 M NaCl, 1.5 mM MgCl
Bacterial cells were fractionated as previously described [ 18 ] . Briefly, bacterial cells were chilled on ice after growth, harvested by centrifugation, and washed in cold phosphate-buffered saline (PBS; [ 34 ] ). Bacterial whole cell fractions were prepared by resuspending the washed cells in cold PBS and precipitating total proteins with 10% (vol/vol) trichloroacetic acid (TCA) on ice overnight.