Example sentences for: resuspending

How can you use “resuspending” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The nuclear proteins were extracted by resuspending the nuclear pellet with buffer C (50 mM Hepes pH7.

  • The pellets were washed once with nuclear buffer, and the nuclear extracts were prepared by resuspending the pellets in 0.3 ml buffer containing 20 mM HEPES (pH 7.8), 25% glycerol, 0.42 M NaCl, 1.5 mM MgCl

  • Bacterial cultures were prepared by resuspending the cells from overnight cultures in 10 mM MgCl 2 to the required optical density (OD 600 = 0.05; 1 OD 600 = 10 9colony-forming units (CFU)/ml).

  • Whole-cell lysis was performed by resuspending the pellet in 2 × SDS sample buffer, vortexing vigorously to shear the chromosomal DNA, and heating to 70°C for about 5 min.

  • Total protein levels in the SDS extracts were determined by BCA assay (Pierce) after removing the reducing reagent from SDS buffer by precipitating protein from the extract with 10% trichloroacetic acid, washing in 10% trichloroacetic acid and resuspending the pellet in 0.1 N NaOH.


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