Words similar to resuspending
Example sentences for: resuspending
How can you use “resuspending” in a sentence? Here are some example sentences to help you improve your vocabulary:
The pellets were washed once with nuclear buffer, and the nuclear extracts were prepared by resuspending the pellets in 0.3 ml buffer containing 20 mM HEPES (pH 7.8), 25% glycerol, 0.42 M NaCl, 1.5 mM MgCl
Total protein levels in the SDS extracts were determined by BCA assay (Pierce) after removing the reducing reagent from SDS buffer by precipitating protein from the extract with 10% trichloroacetic acid, washing in 10% trichloroacetic acid and resuspending the pellet in 0.1 N NaOH.
Cells were harvested, washed four times with 40 ml of ice-cold transport buffer (145 mM NaCl, 1 mM MgCl 2 , 1 mM CaCl 2 , 10 mM glucose, 10 mM HEPES, pH 7.4), titered, and preincubated for 30 min. at 37°C after resuspending 3 × 10 7cells in 1 ml of transport buffer.
The nuclear proteins were extracted by resuspending the nuclear pellet with buffer C (50 mM Hepes pH7.
Listeria were prepared by removing 1 ml of the overnight culture, pelleting by a 1 min Eppendorf centrifuge spin, washing the pellet twice with antibiotic-free media, and resuspending in 1 ml of media.
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