Words similar to resuspending

Example sentences for: resuspending

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Whole-cell lysis was performed by resuspending the pellet in 2 × SDS sample buffer, vortexing vigorously to shear the chromosomal DNA, and heating to 70°C for about 5 min.
Bacterial cells were fractionated as previously described [ 18 ] . Briefly, bacterial cells were chilled on ice after growth, harvested by centrifugation, and washed in cold phosphate-buffered saline (PBS; [ 34 ] ). Bacterial whole cell fractions were prepared by resuspending the washed cells in cold PBS and precipitating total proteins with 10% (vol/vol) trichloroacetic acid (TCA) on ice overnight.
Listeria were prepared by removing 1 ml of the overnight culture, pelleting by a 1 min Eppendorf centrifuge spin, washing the pellet twice with antibiotic-free media, and resuspending in 1 ml of media.
Total protein levels in the SDS extracts were determined by BCA assay (Pierce) after removing the reducing reagent from SDS buffer by precipitating protein from the extract with 10% trichloroacetic acid, washing in 10% trichloroacetic acid and resuspending the pellet in 0.1 N NaOH.
Cells were harvested, washed four times with 40 ml of ice-cold transport buffer (145 mM NaCl, 1 mM MgCl 2 , 1 mM CaCl 2 , 10 mM glucose, 10 mM HEPES, pH 7.4), titered, and preincubated for 30 min. at 37°C after resuspending 3 × 10 7cells in 1 ml of transport buffer.