Words similar to resuspending

Example sentences for: resuspending

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Bacterial cultures were prepared by resuspending the cells from overnight cultures in 10 mM MgCl 2 to the required optical density (OD 600 = 0.05; 1 OD 600 = 10 9colony-forming units (CFU)/ml).
Listeria were prepared by removing 1 ml of the overnight culture, pelleting by a 1 min Eppendorf centrifuge spin, washing the pellet twice with antibiotic-free media, and resuspending in 1 ml of media.
Whole-cell lysis was performed by resuspending the pellet in 2 × SDS sample buffer, vortexing vigorously to shear the chromosomal DNA, and heating to 70°C for about 5 min.
Cells were harvested, washed four times with 40 ml of ice-cold transport buffer (145 mM NaCl, 1 mM MgCl 2 , 1 mM CaCl 2 , 10 mM glucose, 10 mM HEPES, pH 7.4), titered, and preincubated for 30 min. at 37°C after resuspending 3 × 10 7cells in 1 ml of transport buffer.
Total protein levels in the SDS extracts were determined by BCA assay (Pierce) after removing the reducing reagent from SDS buffer by precipitating protein from the extract with 10% trichloroacetic acid, washing in 10% trichloroacetic acid and resuspending the pellet in 0.1 N NaOH.