Words similar to resgen
Example sentences for: resgen
How can you use “resgen” in a sentence? Here are some example sentences to help you improve your vocabulary:
Initially, 2, 4, or 6 ug of RNA was combined with 2 ug of oligo dT (ResGen) and denatured at 70°C for 10 minutes.
Data were collected using a Cyclone Storage Phosphor System (Packard Instruments), and quantified using Pathways 3.0 software (ResGen).
Hybridization of the membrane with radioactive cDNA was performed by the procedures described at http://www.resgen.com/gf200pro.html, except that the last room temperature wash was performed in 2X SSC.
The cDNA was made radioactive by incorporation of 33P-dCTP (ICN #58201) using the procedure described at http://www.resgen.com/gf200pro.html, except that 33 mM of dATP, dTTP, and dGTP (Pharmacia #27-2035-01) and 1 U/μl of a ribonuclease inhibitor (Ambion #2690) were added.
To obtain the full-length MmCOP1 cDNA, an EST clone (GenBank accession number AI509164) containing the N-terminal region of MmCOP1 cDNA in pT7T3D-Pac vector was obtained from ResGen and digested with XhoI and Hind III.
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