Example sentences for: required

How can you use “required” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • However, this is a link to electronic documents available in the Federal Register through the GPO Access web site, not EPA- specific proposed rules, and the viewer is then required to use that site's search tools to identify particular proposed rules.

  • For new commercial chemicals, toxicity testing in animals should be required before they are marketed.

  • An example of a policy was that units were required to use commercially developed software rather than developing unique software inhouse.

  • To determine if extracellular Ca 2+is also required for MTX-induced membrane blebbing, changes in cell morphology were correlated with ethidium uptake in the presence of Ca 2+-free extracellular buffer (Fig.

  • Several lines of evidence suggest that the SCF pathway is also conserved in the fission yeast Schizosaccharomyces pombe . Pcu1p, a Cdc53p/CUL1-related protein, was shown to associate with two different F-box/WD repeat proteins, Pop1p and Pop2p, when overexpressed [ 18 ] . In addition, genetic studies demonstrated that both of these F-box proteins are simultaneously required for efficient destruction of Rum1p and the replication initiator Cdc18p [ 18 19 20 ] . Rum1p is a Sic1p-analogous CDK inhibitor, which accumulates in G1, but is degraded as cells enter S phase [ 21 22 ] . Failure to degrade Rum1p is the major phenotypic defect of pop1 and pop2 deletion strains, which leads to disturbance of normal cell cycle progression, resulting in polyploidy [ 18 19 20 23 ] . Based on the genetic data and the biochemical observation that Pop1p and Pop2p interact when overexpressed, a putative SCF Pop1p-Pop2pcomplex was proposed, which would contain the heterooligomerizing F-box proteins Pop1p and Pop2p bound to SCF core components [ 18 20 ] .


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