Words similar to replication-deficient

Example sentences for: replication-deficient

How can you use “replication-deficient” in a sentence? Here are some example sentences to help you improve your vocabulary:

The adenovirus (AV) backbone for the aquaporin-1 (AQP1) sense and antisense AV constructs was a replication-deficient "first-generation" AV with deletions of the E1 and E3 genes [ 26 ] . This "empty" AV contains the cytomegalovirus (CMV) promoter and bovine growth hormone polyadenylation (bHG) site separated by a polylinker that was used to clone AQP1 DNA as described previously [ 27 ] . A recombinant AQP1 virus was constructed using a plasmid containing the coding sequence for AQP1, pCHIPev [ 28 ] . pCHIPev was digested and the AQP1 insert was subcloned into the shuttle vector pSKAC, creating pSKAC/AQP1.
MDCK cells were induced to express AQP1 by infection with replication-deficient adenovirus (see Materials and Methods).
The recombinant adenoviral vector used in the present study originates from replication-deficient type 5 adenovirus lacking E1 and E3 loci [ 21 ] . The human TGF-β1 cDNA was inserted in place of the E1 region in the shuttle plasmid pAd-Lox [ 22 ] , where expression is driven by the cytomegalovirus promoter.