Words similar to replication-defective
Example sentences for: replication-defective
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Cortical astrocytes were harvested from neonatal C57BL/6 mice and infected with a replication-defective retrovirus encoding Tag and neomycin resistance as previously described [ 13 ] . The transferred viruses were SV40-6 [ 5 ] , in which wild type Tag is expressed from the M-MuLV LTR promoter; SV(X)T K1, which is constructed the same as SV40-6 except that the Tag is mutant [ 38 ] and defective for binding the retinoblastoma protein family [ 39 ] ; Linker-tkT, in which the cDNA for Tag is expressed from the herpes simplex 1 tk promoter [ 11 ] . The differentiation and transformation phenotypes of the cell lines utilizing the M-MuLV LTR promoter have been described [ 6 13 ] . The Linker-tkT lines (tkT) were either isolated as clones after G418 selection (as previously described) [ 6 ] or frozen immediately after selection (pooled clones) and clonally selected at a later time.
The adenoviral vector DNA were linerized with Pac I and transfected into the replication-permissive 293 cells (E1A transcomplementing cell line) using Lipofectamine (Life Technologies) to produce E1-deleted, replication-defective recombinant adenovirus as described previously [ 30 ] . Large-scale amplification of the recombinant adenovirus in 293 cells was followed by purification using a discontinuous CsCl gradient.
Gene trap retroviruses developed in our laboratory contain a selectable marker in the U3 region of the long terminal repeat (LTR) of a replication-defective Moloney murine leukemia virus.
Replication-defective adenoviral vectors offer several advantages that make them attractive for gene delivery [ 3 4 5 6 ] . Adenoviral vectors are able to transduce proliferating or quiescent cells; they can accommodate dual, large insert sizes and can deliver additional genes by superinfection; and stable, high-titer viral stocks are easily generated [ 7 ] . Further, since the adenoviral genome is maintained extra-chromosomally, insertion site-dependent effects of the host genome are minimized, and exogenous genes can be reliably expressed at high levels.
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