Example sentences for: reconstitute

How can you use “reconstitute” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Given our ability to prepare immunopurified SCF Pop1p-Pop2pthat bound phosphorylated Rum1p, we sought to reconstitute Rum1p polyubiquitylation in vitro.

  • Complete repair at some of these steps would reconstitute a circular chromosome, which will stay in the well.

  • In addition, we have found that kinase inactive Btk can reconstitute BCR-induced ERK MAPK activation, but not PLCγ2 phosphorylation (Tomlinson, Bolen and Johnston, unpublished data).

  • The RNA genome of RSV is about 15 kb long and contains 11 documented protein-coding genes [ 13 ] . Three viral proteins are minimally required to reconstitute the functional transcription complex of NNR viruses [ 3 ] : the nucleocapsid protein (N) that wraps the negative-strand genome RNA and its full-length complement, the positive-strand antigenome RNA, thus converting them into highly nuclease-resistant, chromatin-like templates; the large protein (L), which is the major subunit of the RdRP; and the phosphoprotein (P), which is the smaller subunit of RdRP and an essential transcription factor of L [ 19 20 21 22 ] . In RSV, optimal transcription, although not replication, additionally requires the transcription antitermination protein M2-1 [ 13 ] . In addition, cellular actin, and to a lesser extent, profilin, are also required for viral transcription [ 17 18 ] .

  • The recE gene product of the Rac prophage converts dsDNA ends into 3' protruding single-stranded form and the recT gene product promotes recombination by annealing them with a homologous DNA in its vicinity (for review, see [ 7 8 ] ). This recombination may result in one progeny DNA (non-conservative recombination) or two progeny DNAs (conservative double-strand break repair) [ 9 ] . In a recBC sbcBC background, a ds end stimulates homologous recombination that results in only one progeny DNA (non-conservative recombination) [ 10 ] . Analysis of the stimulation of recombination by replication (for review, see [ 11 ] ) and analysis of altered chromosomal replication (for review, see [ 12 ] ) led to the proposal that a chromosomal ds break formed during replication fork arrest triggers homologous recombination, which would reconstitute a replication fork (for review, see [ 5 ] ).


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