Example sentences for: rcf
How can you use “rcf” in a sentence? Here are some example sentences to help you improve your vocabulary:
cfu = colony forming units; COX-2 = cyclooxygenase-2; DMEM = Dulbecco's modified Eagle's medium; EGFP = enhanced green fluorescent protein; FACS = fluorescence-activated cell sorting; FLS = fibroblast-like synovial cells; MoMLV = Moloney murine leukemia virus; PCR = polymerase chain reaction; RA = rheumatoid arthritis; RCF = relative centrifugal force.
The expression data also suggested that centrifugation at a RCF as high as 30,000 g for four hours did not affect viability of the recombinant retrovirus.
Our studies indicate that the time necessary to recover essentially all viral particles can be reduced to four hours by increasing the RCF to 20,000 g . The protocol does not appear to adversely affect the infectivity of the viral preparation, as the functional viral titer on NIH 3T3 cells closely matched the titer that was predicted by the degree of concentration.
We observed a progressive rise in viral titer as RCF was increased from 6000 to 20,000 g . At a RCF of 20,000 g , the titer of the resuspended pellet reached a plateau value of 1.3 × 10 8cfu/ml.
Concentrated recombinant retrovirus, generated by super-speed centrifugation of retroviral supernatant, has been used to improve the transduction efficiency of primary cells, including hepatocytes [ 9 ] and endothelial cells [ 11 ] . In these prior reports, concentration was accomplished by centrifugation for 16 hours at a RCF of 6000 g . We used a recombinant retrovirus encoding the green fluorescent protein to optimize a protocol to rapidly and efficiently concentrate retrovirus by superspeed centrifugation.
Loading...