Example sentences for: rcf
How can you use “rcf” in a sentence? Here are some example sentences to help you improve your vocabulary:
Further concentration of viral particles was not achieved by increasing RCF above 20,000 g . Viral particles were not detectable by expression assay or by slot blot hybridization analysis in the post-centrifugation supernatant at an RCF of 20,000 g or higher.
Concentrated recombinant retrovirus, generated by super-speed centrifugation of retroviral supernatant, has been used to improve the transduction efficiency of primary cells, including hepatocytes [ 9 ] and endothelial cells [ 11 ] . In these prior reports, concentration was accomplished by centrifugation for 16 hours at a RCF of 6000 g . We used a recombinant retrovirus encoding the green fluorescent protein to optimize a protocol to rapidly and efficiently concentrate retrovirus by superspeed centrifugation.
As the de facto rules of Internet use, RCF 1855 should be read by all new Internet users--including Slate writers.
The time of centrifugation was fixed at four hours and the RCF was varied in a range from 6000 to 30,000 g . Following centrifugation, the viral pellet was resuspended in a hundredth of the original volume.
The expression data also suggested that centrifugation at a RCF as high as 30,000 g for four hours did not affect viability of the recombinant retrovirus.