Words similar to rapamycin-sensitive
Example sentences for: rapamycin-sensitive
How can you use “rapamycin-sensitive” in a sentence? Here are some example sentences to help you improve your vocabulary:
In cultured human muscle cells, only the PI 3-kinase - p70 S6 kinase pathway, and not the MAPK pathway, mediates insulin up-regulation of p85α PI 3-kinase gene expression [ 26 ] . Similarly, in L6 myotubes, insulin induces hexokinase II gene transcription via the rapamycin-sensitive PI 3-kinase pathway, but not via the MAPK pathway [ 27 ] . The PI 3-kinase - p70 S6 kinase pathway, but not the MAPK pathway, mediates insulin induction of glucose-6-phosphate dehydrogenase gene expression in primary rat hepatocytes [ 28 ] . In H411E liver cells, insulin down-regulates cAMP-induced phosphoenolpyruvate carboxykinase gene transcription via the PI 3-kinase pathway, but not via the MAPK pathway [ 29 ] . Phosphorylated p70 S6 kinase has been shown to be present in the nucleus in insulin-treated cells [ 30 ] . Moreover, it has been shown that p70 S6 kinase phosphorylates and activates members of the cAMP response element modulator family of transcription factors [ 31 ] . These data raise the possibility that transcriptional regulation of SP-A gene expression occurs via the PI 3-kinase - p70 S6 kinase pathway.
Our studies are suggestive that insulin inhibits SP-A gene transcription via the PI 3-kinase pathway in lung epithelial cells, specifically via the rapamycin-sensitive activation of p70 S6 kinase.
We confirmed these observations by showing that insulin increased the phosphorylation of a specific rapamycin-sensitive residue (threonine 389), present in the linker region of p70 S6 kinase, a phosphorylation event that is known to activate this enzyme [ 22 ] . In contrast, PD98059, an inhibitor of p44/42 MAPK activation, did not block the inhibitory effects of insulin on SP-A gene expression.
Together, our results are suggestive that insulin activates the rapamycin-sensitive PI 3-kinase pathway in lung epithelial cells and that this event leads to an inhibition of SP-A gene transcription.
In summary, our studies are suggestive that in lung epithelial cells, SP-A gene transcription is inhibited by insulin via the rapamycin-sensitive PI 3-kinase pathway, but not the p44/42 MAPK pathway.
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