Words similar to r-pcr

Example sentences for: r-pcr

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First, yields from IVT are significantly higher than those obtained using R-PCR (Table 1), with a fold-amplification similar to that obtained in mRNA linear amplification protocols [ 16 17 ] . Since 2 μg of amplified RNA are sufficient to generate probe for a microarray experiment, RNA from a single IVT amplification can be used for several analyses.
We compared our IVT protocol to a commonly used PCR protocol that uses a partially degenerate primer to amplify genomic DNA in a sequence independent manner (R-PCR).
The direct labeling and R-PCR protocols are also highly reproducible, with mean correlations of 0.93 and 0.91, respectively (Fig 3A).
Hence, we used direct labeling as a 'gold standard' to validate the IVT and R-PCR methods.
The IVT yielded almost 80 μg RNA, while the R-PCR yielded 12 μg DNA (Table 2).