Example sentences for: r-pcr
How can you use “r-pcr” in a sentence? Here are some example sentences to help you improve your vocabulary:
Hence, we used direct labeling as a 'gold standard' to validate the IVT and R-PCR methods.
We find that this protocol generates higher yields and better maintains the size distribution of the starting material than an alternative R-PCR approach.
First, yields from IVT are significantly higher than those obtained using R-PCR (Table 1), with a fold-amplification similar to that obtained in mRNA linear amplification protocols [ 16 17 ] . Since 2 μg of amplified RNA are sufficient to generate probe for a microarray experiment, RNA from a single IVT amplification can be used for several analyses.
R-PCR samples were generated from 50 ng restricted DNA using the Round A/ Round B protocol described in [ 9 12 ] . Briefly, Sequenase (Amersham) was used for two cycles of synthesis using a degenerate primer (5'-GTTTCCCAGTCACGATCNNNNNNNNN-3' ('round A').
Correspondingly, the highest ranking features in the direct labeling dataset overlap extensively with the highest ranking features in the IVT dataset and, to a lesser extent, with the highest ranking features in the R-PCR dataset (Fig 3B).