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Example sentences for: qiaquick

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Cy3- and Cy5-labeled cDNA targets were then purified on QIAquick PCR purification columns, combined and concentrated by lyophilization, and hybridized to the microarray at 42°C for 16 h in hybridization solution containing 50% formamide, 5x SSC, 0.1% SDS, 20 μg mouse Cot-1 DNA and 10 μg poly(dA).
For selected samples, the amplified DNA product from a single 100-μl reaction was purified with the QIAquick PCR Purification Kit (Cat.
The reactions were thermocycled 15 times at 94°C for 30 seconds, 45°C for 30 seconds, 72°C for 1 minute and then purified with a QIAquick PCR purification kit (Qiagen, Valencia, CA).
The cDNA:mRNA hybrid was isolated on a Qiaquick PCR purification column (Qiagen) and degraded after addition of 2.5 μl 1 N NaOH for 10 minutes at 37°C.
The agarose plug was added to a fresh PCR reaction (100 μl) and cycled 15 times; DNA was purified using a QIAquick kit and quantitated.