Words similar to qiaquick
Example sentences for: qiaquick
How can you use “qiaquick” in a sentence? Here are some example sentences to help you improve your vocabulary:
The reactions were thermocycled 15 times at 94°C for 30 seconds, 45°C for 30 seconds, 72°C for 1 minute and then purified with a QIAquick PCR purification kit (Qiagen, Valencia, CA).
A total of 35 cycles (94°C/20 sec; 56°C/20 sec; 72°C/1 min) was used to amplify both exons 2 and 3. PCR amplification products were purified using the QIAquick PCR Purification Kit (Qiagen) and both strands were sequenced on an ABI automated sequencer.
The agarose plug was added to a fresh PCR reaction (100 μl) and cycled 15 times; DNA was purified using a QIAquick kit and quantitated.
Each double-stranded cDNA sample was purified by sequential phenol/chloroform extraction (Ambion, Austin, TX) and adsorption to silica (Qiaquick™ kit, Qiagen, Hilden, Germany) according to manufacturers' instructions.
Reactions were neutralized with 10 μl of 1 M HCl, and cDNA was purified on QIAquick columns (Qiagen) according to the manufacturer's directions but substituting phosphate wash buffer (5 mM potassium phosphate pH 8.0, 80% ethanol) for buffer PE, and phosphate elution buffer (4 mM potassium phosphate pH 8.5) for buffer EB.