Words similar to qiagen

Example sentences for: qiagen

How can you use “qiagen” in a sentence? Here are some example sentences to help you improve your vocabulary:

Reactions were neutralized with 10 μl of 1 M HCl, and cDNA was purified on QIAquick columns (Qiagen) according to the manufacturer's directions but substituting phosphate wash buffer (5 mM potassium phosphate pH 8.0, 80% ethanol) for buffer PE, and phosphate elution buffer (4 mM potassium phosphate pH 8.5) for buffer EB.
After RNA amplification, the DNA template was removed by incubation of the reaction with 4 units of RNase-free DNase I (Ambion) at 37°C for 15 min. aRNA purification was achieved by Rneasy Mini Kit (Qiagen).
Transfections were performed with Superfect reagent according to the manufacturer's protocol (Qiagen, Valencia, CA).
The labeled DNA was mixed with 50 μl of 100 mM sodium acetate (pH 5.2), purified with Qiagen PCR Purification Kit, reduced to a final volume of 7.5 μl with a speed vac, mixed with 3.75 μl of 20x SSC, 0.75 μl of 10% SDS, 1.5 μl of 1 μg/μl salmon DNA and 1.5 μl of 50x Denhardt's solution, denatured at 95°C for 5 min, cooled on ice and incubated at 42°C for 15 min.
For both EGFR and KRAS, PCR was performed using the HotStarTaq Master Mix Kit (Qiagen, Valencia, California, United States), as per manufacturer's instructions.