Words similar to qiagen
Example sentences for: qiagen
How can you use “qiagen” in a sentence? Here are some example sentences to help you improve your vocabulary:
Cells were transfected with the indicated expression vectors, using Lipofectamine Plus reagent (Life Technologies, Inc., Rockville, MD) for 293 cells or Superfect reagent (Qiagen, Valencia, CA) for 3T3 cells, according to the protocols recommended by the manufacturers.
For each amplicon, 1μl of cDNA, 1.5μl of 10 × PCR buffer (Qiagen), 250μM of each dNTP, 0.5 units of HotstarTaq polymerase (Qiagen) and 0.5μM of primer (Life Technologies) were used in a 15μl reaction.
RNA from a colon tumor liver metastasis was extracted with Trizol (Life Technologies) and further purified on RNeasy columns (Qiagen) using the protocol supplied by the manufacturers.
After centrifugation at maximum speed for 15 minutes, the supernatant was incubated with Ni-NTA resin (Qiagen) for 1.5 hour at room temperature.
The Cy3 (HL60) and Cy5 (HL60/TLK286) PCR products were purified with Qiagen PCR purification kit, reduced to a final volume of 7.5 μl with a speed vac, mixed with 3.75 μl of 20x SSC, 0.75 μl of 10% SDS, 1.5 μl of 1 μg/μl salmon DNA and 1.5 μl of 50x Denhardt's solution, denatured at 95°C for 5 min, cooled on ice and incubated at 42°C for 15 min.