Example sentences for: qiagen

How can you use “qiagen” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • First-strand cDNA was purified from unincorporated amino allyl-dUTPs on QIAquick PCR purification columns (Qiagen, Valencia, CA) according to manufacturer's instructions, except that QIAquick wash buffer was replaced with 5 mM K +phosphate buffer (pH 8.5) containing 80% ethanol, and cDNA was eluted with 4 mM K +phosphate buffer (pH 8.5).

  • Purification of cDNA was done using the Qiagen PCR Purification Kit, and cDNA concentration was measured by spectrophotometry.

  • Run-off PCR was performed as described above except that only five cycles of PCR were run with the 55 °C annealing temperature and QuantiTect Probe PCR kit (Qiagen).

  • IVT cleanup was performed using Rneasy ®Columns (Qiagen, Valencia, CA).

  • The amplification products were purified using a Qiaquick PCR purification spin column kit (Qiagen, Valencia CA).


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