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Example sentences for: purified
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We also found that HA-NFATp could be purified from cell extracts by DNA affinity chromatography using an immobilized double stranded oligonucleotide containing two high affinity NFAT binding sites, followed by elution with NaCl (Figure 1C).
Total RNA from young tomato leaves ( Lycopersicon esculentum ) was isolated as described by Wanner and Gruissem [ 44 ] . mRNA was purified using the mRNA purification kit (Pharmacia Biotech, Piscataway, NJ).
Recombinant protein was purified from bacterial lysates in accordance with the published method for the QIA express system (Qiagen, Valencia, CA, USA) with variations in Buffers B and D as noted in Additional file: 1. Procedures were carried out at 4°C; dialyzed recombinant DEK protein (rDEK) was stored at -80°C.
Consistent with our microarray results on CCR5 up-regulation, experiments performed in peripheral blood mononuclear cells (PBMCs) with soluble Tat has shown selective entry and replication of CCR5 virus into cells [ 27 28 ] . Up-regulation of HIV-1 coreceptor by Tat has also been reported, where a synthetic Tat protein that was immobilized on a solid substrate, up-regulated the surface expression of the chemokine receptors in purified populations of primary resting CD4+ T cells.
Previous reports of HPLC assays for ALDH reactions with tissue extracts used a single time point at 60 min to measure accumulation of product, after documenting that retinoic acid formation was still linear in time course experiments [ 33 ] . In our hands, the reaction with purified enzyme was only linear for product formation up to 10-15 minutes.
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