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Example sentences for: purified
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Human thymocytes were purified from fresh residual thymic tissue that was obtained as an incidental specimen from immunologially normal pediatric patients undergoing cardiac surgery and according with institutional guidelines.
A total of 35 cycles (94°C/20 sec; 56°C/20 sec; 72°C/1 min) was used to amplify both exons 2 and 3. PCR amplification products were purified using the QIAquick PCR Purification Kit (Qiagen) and both strands were sequenced on an ABI automated sequencer.
The mouse homologue of one of the candidate biomarkers, LNC2, has been shown to be regulated at the transcriptional level by dexamethasone in murine cells [ 22 ] . However, there is no published evidence that the human LCN2 gene is likewise dexamethasone-inducible, and there is no description of glucocorticoid-responsive elements in the 5' regulatory region of the human LCN2 gene [ 28 ] . Further, preliminary investigation in our laboratory, using in vitro cultures of purified PBMCs from healthy volunteers, indicates that neither the LCN2 gene nor any of the other 3 transcripts is directly inducible by acute treatment with either SU5416 or dexamethasone (data not shown).
In fact, in vitro motility studies with a purified myosin from Chara (myosin XI, Cc ccm in Figure 3) have confirmed that it is indeed an actin-based motor [ 54].
After evaporation of ethyl acetate, purified siderophore was re-suspended in distilled water and stored at -80°C.