Words similar to provirus
Example sentences for: provirus
How can you use “provirus” in a sentence? Here are some example sentences to help you improve your vocabulary:
The present study characterized a mutation in the 1B4 cell line induced by the U3Neo gene trap retrovirus [ 29 ] . This insert was selected for study because the provirus inserted into a widely expressed gene and yet no phenotype was observed in mice homozygous for the provirus.
Therefore the 1B4 provirus appeared to integrate 159 nucleotides into an intron of the hnRNP A2/B1 gene.
First, two primers complementary to hnRNP A2/B1 sequences located upstream and downstream of the provirus were used together with a neo specific primer in separate PCR reactions.
adjacent to the 1B4 provirus were isolated by inverse polymerase chain reaction (PCR) as reported elsewhere [ 29 ] . This flanking sequence was used as a probe to genotype mutant mice and cells by Southern blot hybridization and was also used to isolate cDNA clones encoding the murine hnRNP A2/B1 protein from a PCC3 embryonal carcinoma cell cDNA library.
The present study identified a mechanism that allows expression of a U3Neo gene from a provirus positioned within an intron.
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