Example sentences for: promoter

How can you use “promoter” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The selection was successfully employed to identify a mutant T7 RNA polymerase 'specialist' which could recognize and transcribe a T3-like promoter, a polymerase 'generalist' which was able to recognize a variety of T7 promoters, and a novel RNA polymerase with an apparently new promoter specificity.

  • An amplicon containing the hsp65 promoter and full-length Rv0365c gene was generated by PCR amplification of the pHIP2 plasmid.

  • The gross structural features of the collected Runx genes, including positions of exons and introns (excepting those upstream of the proximal promoter of the vertebrate Runx genes - see below), are depicted schematically in Figure 1(see also Supplemental Table 2and Supplemental Figure 1).

  • Where indicated recombinant NFATp proteins were pre-incubated with promoter DNA for five min at 30°C prior to the addition of the remaining general transcription factors (TFIIA, TFIIB, TFIID, TFIIE, TFIIF, and TFIIH) and RNA polymerase II.

  • The generation of Hoxc-8 transgenic mice and the characterization of their phenotype have been published [ 1 ] . Briefly, Hoxc-8 expressing mice are generated in a binary transgenic mouse system [ 44 ] by crossing a transactivator strain (harboring a transgene in which expression of the transcriptional transactivator VP16 is under control of the Hoxc-8 promoter; [ 45 ] ) to a transresponder strain (that carries as a transgene the Hoxc-8 cDNA linked to the ICP4 immediate early gene promoter, which is silent in mice in the absence of VP16; [ 45 46 ] ). Upon combination of both transgenes, the Hoxc-8 transresponder transgene is activated, and Hoxc-8 becomes overexpressed [ 23 ] . This system also allows the generation of transgenic mice with increased gene dosage by superimposition of transgene loci to homozygosity [ 23 44 ] . For the studies reported here, we used the T239 transactivator and the IE-c8-254 transresponder strains.


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