Words similar to prohb-egfex
Example sentences for: prohb-egfex
How can you use “prohb-egfex” in a sentence? Here are some example sentences to help you improve your vocabulary:
Y190 transformants containing pGBT9/proHB-EGFEx and pGAD10/cv-1 library were plated onto Trp -Leu -His -agar plates.
The pGBT9/proHB-EGFEx construct was used as the bait construct in the yeast two-hybrid screen to identify extracellular proteins that interact with the extracellular domain of proHB-EGF.
Each construct from the cDNA library that tested positive for both lacZ and his reporter gene expression was also tested for the ability to activate directly β-galactosidase/ his reporter gene transcription without the presence of the bait construct, pGBT9/proHB-EGFEx.
The PCR product was digested with Eco RI and Bam HI and ligated into the Eco RI and Bam HI restriction sites of pGBT9 and pGAD424 to produce constructs pGBT9/proHB-EGFEx and pGAD424/proHB-EGFEx, respectively.