Words similar to priming
Example sentences for: priming
How can you use “priming” in a sentence? Here are some example sentences to help you improve your vocabulary:
The T7-(dT) 24 oligomer was used for priming first-strand cDNA synthesis.
Both the addition of IL-4 to the priming cultures and the presence of anti-IFN-γ antibody ensured that potential IL-4-secreting precursor populations had optimal conditions for differentiation.
Priming with various types of "live" expression followed by boosting with purified subunits is currently favored as a method to obtain stronger immunologic responses that either method alone [reviewed in [ 1 ] and [ 2 ] ]. Previously acquired immunity to a viral vector such as vaccinia may influence its efficacy in inducing immunity against heterologous proteins being delivered [ 16 17 18 19 ] and it may be wise to provide physicians with HIV vaccines based on a variety of vectors to handle a variety of clinical situations.
Synthesis of cDNA from the cRNA initially prepared is expected to lead to some truncation of the 5' ends of the original mRNA, due to the requirement for priming during synthesis of the second strand.
A widely used protocol, developed by Eberwine and colleagues, uses T7-based linear amplification [ 14 ] . In this procedure, double stranded cDNAs are synthesized from mRNA by reverse-transcription (priming off the 3' polyA tail) and second strand synthesis.
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