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Example sentences for: previous

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Lysates were immunoprecipitated with minor alterations to previous protocols [ 8 ] . Specifically, the IP buffer contained only 50 mM NaCl and incubation with the primary antibody was performed overnight at 4°C.
Previous studies on rats have shown that adults of some strains can be made reproductively responsive to SD by manipulations such as androgen treatment [ 8 9 ] , olfactory bulbectomy [ 21 22 ] (but see [ 23 ] ), or food restriction [ 7 ] . An effect of short photoperiod on body weight of unmanipulated adult rats has not been reported previously, but has been reported for unmanipulated young rats of several strains (Zucker rats [ 24 ] , F344 rats [ 5 15 ] , Wistar rats [ 25 ] , and BN rats [ 6 ] ). To our knowledge, this study is the first report of reproductive and nonreproductive responses to short photoperiod in adult, unmanipulated rats.
Our previous studies on animal models have shown that intercalated duct cells in adult rat submandibular glands are the common progenitor for both the acinar and granular duct cells [ 27 36 ] . A recent study in human breast has revealed that CK5-positive cells are capable of giving rise to both glandular and ME cell lineages [ 21 ] . In both cases the intermediate cells showed an unusual immunostaining pattern for several proteins [ 21 36 ] .
The average duration of any breastfeeding in the mothers' previous children was (months ± SD): All children: 16.
In a previous study, Van Compernolle et al.