Words similar to pret
Example sentences for: pret
How can you use “pret” in a sentence? Here are some example sentences to help you improve your vocabulary:
To provide confirmation that improved transduction of FLS was associated with an increase in the intracellular expression of a virally encoded transgene, FLS were transduced with a vector encoding human COX-2 (pRET2.COX2).
The successful transinfection of pRET2.COX2 into PA317 was confirmed by G418 selection.
The EGFP cDNA was PCR amplified from pEGFP-1 (Clontech, Palo Alto, CA, USA) and subcloned into pRET2, a modified version of the MoMLV-based MFG retroviral vector, designed to optimize gene expression in primary cell lines.
Following transinfection with ecotropic viral supernatant, 100% of the PA317 cells were transduced with the pRET2.EGFP vector, as determined by fluorescence microscopy.
The pRET2.EGFP or pRET2.COX2 plasmids were transfected into ecotropic Phoenix E packaging cells by the calcium phosphate precipitation method, using the ProFection kit (Promega, Madison, WI, USA).