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Example sentences for: pret
How can you use “pret” in a sentence? Here are some example sentences to help you improve your vocabulary:
The successful transinfection of pRET2.COX2 into PA317 was confirmed by G418 selection.
The EGFP cDNA was PCR amplified from pEGFP-1 (Clontech, Palo Alto, CA, USA) and subcloned into pRET2, a modified version of the MoMLV-based MFG retroviral vector, designed to optimize gene expression in primary cell lines.
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The pRET2.EGFP or pRET2.COX2 plasmids were transfected into ecotropic Phoenix E packaging cells by the calcium phosphate precipitation method, using the ProFection kit (Promega, Madison, WI, USA).
The pRET2 incorporates long-terminal repeats from the myeloproliferative sarcoma virus [ 7 ] , and a point mutation in the primer binding site [ 8 ] . A vector expressing the human cyclooxygenase-2 (COX-2) cDNA was constructed in the same backbone (pRET2.COX2).