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Example sentences for: pret
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To provide confirmation that improved transduction of FLS was associated with an increase in the intracellular expression of a virally encoded transgene, FLS were transduced with a vector encoding human COX-2 (pRET2.COX2).
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Following transinfection with ecotropic viral supernatant, 100% of the PA317 cells were transduced with the pRET2.EGFP vector, as determined by fluorescence microscopy.
The pRET2.EGFP or pRET2.COX2 plasmids were transfected into ecotropic Phoenix E packaging cells by the calcium phosphate precipitation method, using the ProFection kit (Promega, Madison, WI, USA).
The pRET2 incorporates long-terminal repeats from the myeloproliferative sarcoma virus [ 7 ] , and a point mutation in the primer binding site [ 8 ] . A vector expressing the human cyclooxygenase-2 (COX-2) cDNA was constructed in the same backbone (pRET2.COX2).
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