Words similar to prephosphorylated

Example sentences for: prephosphorylated

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Following incubation in each of the primary kinases, the prephosphorylated p53-GST was then incubated with 32P-ATP and either GSK3α or GSK3β.
p53-GST was then prephosphorylated with MAP kinase, S6 kinase, Protein Kinase A, DNA-PK and Protein Kinase C (fig 1) or Casein Kinase II and JNK1/JNK2 (data not shown).
Aliquots of the prephosphorylated p53-GST were then incubated with or without recombinant GSK3α or GSK3β and 32P-ATP to measure p53 phosphorylation.
Unphosphorylated p53-GST was not a substrate for GSK3β, whereas p53-GST prephosphorylated by DNA-PK was (fig 2).
I-1 was prephosphorylated by PKA in a standard kinase reaction containing 200 μM γ-thiophosphorylated ATP.