Words similar to prephenate
Example sentences for: prephenate
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Thus, Anabaena has two additional extra-operonic paralogs of aroA Iβ and trpD . In addition to extra-operonic, free-standing copies of trpAa and trpAb , a second fused gene ( trpAa • trpAb_2 ) encoding the two domains of anthranilate synthase is present in Anabaena . Nostoc has two extra-operonic copies of aroA Iβ , aroB and trpD . All cyanobacteria possess AroA of the Iβ class ( aroA Iβ ). While this is also true of the Anabaena/Nostoc lineage (in fact, having multiple copies), both Anabaena and Nostoc possess an additional gene encoding AroA of the Iα class ( aroA Iα ). All cyanobacteria possess a tyrA gene of the arogenate-specificity class ( tyrA a ), but the Anabaena/Nostoc supraoperons also possess a tyrA gene deemed to be a cyclohexadienyl dehydrogenase [ 28] with a favored specificity for prephenate ( tyrA (p) ) (C.A.B.
*PheC [ 16] activity (assayed as prephenate dehydratase) was the marker protein for the periplasmic fraction [ 13], and shikimate dehydrogenase activity was the marker protein for the cytoplasmic fraction.
For routine assays with unpurified protein preparations, chorismate mutase and prephenate dehydratase were assayed spectrophotometrically by the method of Cotton and Gibson [ 38] as modified by Ahmad and Jensen [ 18], using 1 mM chorismate and 1 mM prephenate, respectively.
Chorismate, prephenate, and L-arogenate are routinely prepared in this laboratory as the accumulated products of mutant microbial strains.
In the wild-type, the prephenate dehydratase activity of the P-protein is completely inhibited by phenylalanine, whereas *PheC is completely insensitive.