Words similar to precoated

Example sentences for: precoated

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Peripheral blood mononuclear cells (PBMCs) were incubated overnight at 50,000 to 100,000 cells/well in 96-well polyvinylidene plates that had been precoated with 0.5 μg/ml anti-human interferon-γ monoclonal antibody (Mabtech, Stockholm, Sweden).
One hundred microliters of RNA solution diluted to 2.5 ng/ μl was added to an appropriate number of wells in a 96-well microtiter plate precoated with Streptavidin (Pierce) and incubated for 1 hr at room temperature.
Cells were then plated onto precoated dishes and processed for immunoblotting with anti-phospho-p44/42 MAPK antibody as described above.
Evidence for CAP-HIV-1 binding was obtained from results of solid phase immunoassays in which attachment of HIV-1 IIIB and BaL virus particles, respectively (detected by subsequent ELISA of p24 antigen released from virus particles by detergent treatment) to wells precoated with CAP was measured (Fig.
Sympathetic neurons were dissociated from the SCG of perinatal (E21 to PN1) Holtzmann rats (Harlan Sprague-Dawley, Rockford, IL) according to previously described methods [ 59 ] . Cells were plated onto glass coverslips (for immunocytochemical and morphological studies) or 35 mm plastic culture dishes (for Western blot analyses) precoated with 100 μg/ml poly-D-lysine (Sigma, St. Louis, MO).