Example sentences for: precoated

How can you use “precoated” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • One hundred microliters of RNA solution diluted to 2.5 ng/ μl was added to an appropriate number of wells in a 96-well microtiter plate precoated with Streptavidin (Pierce) and incubated for 1 hr at room temperature.

  • Evidence for CAP-HIV-1 binding was obtained from results of solid phase immunoassays in which attachment of HIV-1 IIIB and BaL virus particles, respectively (detected by subsequent ELISA of p24 antigen released from virus particles by detergent treatment) to wells precoated with CAP was measured (Fig.

  • Sympathetic neurons were dissociated from the SCG of perinatal (E21 to PN1) Holtzmann rats (Harlan Sprague-Dawley, Rockford, IL) according to previously described methods [ 59 ] . Cells were plated onto glass coverslips (for immunocytochemical and morphological studies) or 35 mm plastic culture dishes (for Western blot analyses) precoated with 100 μg/ml poly-D-lysine (Sigma, St. Louis, MO).

  • Cells were then plated onto precoated dishes and processed for immunoblotting with anti-phospho-p44/42 MAPK antibody as described above.

  • Peripheral blood mononuclear cells (PBMCs) were incubated overnight at 50,000 to 100,000 cells/well in 96-well polyvinylidene plates that had been precoated with 0.5 μg/ml anti-human interferon-γ monoclonal antibody (Mabtech, Stockholm, Sweden).


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