Words similar to pre-mi
Example sentences for: pre-mi
How can you use “pre-mi” in a sentence? Here are some example sentences to help you improve your vocabulary:
This suggested that miRNA genes might be found by folding fixed lengths of conserved sequence to identify ones that display the high degree of relatively continuous helicity characteristic of known pre-miRNAs.
Processing of pri-miRNA into the pre-miRNA stem-loop occurs in the nucleus, while subsequent processing of pre-miRNA into 21-22 mers is a cytoplasmic event mediated by the RNAse III enzyme Dicer [ 17 18 19 20 ] ; Dicer is also responsible for cleavage of long perfectly double-stranded RNA into 21-22 nucleotide fragments during RNA interference (RNAi) [ 2 21 ] . These latter molecules, known as silencing RNA (siRNA), bind to and trigger the degradation of perfectly homologous mRNA molecules via RISC, a double-strand RNA-induced silencing complex containing nuclease activity [ 22 23 ] .
The starting point for our studies was a reference set of 24 Drosophila pre-miRNA sequences ( let-7 , the 21 originally identified by Lagos-Quintana and colleagues, mir-125 , and a previously undescribed paralog of mir-2 that we named mir-2c [ 9 10 29 ] ). We analyzed this set to derive rules and determine parameters that specifically describe known miRNA genes within anonymous genomic sequence.
Consideration of nucleotide bias at the 5' end of miRNA (including a propensity to begin with U) may improve predictions [ 11 ] , although we stress that our algorithms were designed to predict pre-miRNA genes and not mature miRNA sequences themselves.
The size of the hairpin loop was not specifically evaluated as it appears to be variable in known pre-miRNAs; however it was indirectly assessed, as maximization of stem length concomitantly minimizes terminal loop size.
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