Words similar to pre-incubated
Example sentences for: pre-incubated
How can you use “pre-incubated” in a sentence? Here are some example sentences to help you improve your vocabulary:
These compounds were pre-incubated with the virus and then the mixture was inoculated on CV-1 cells and residual virus titers were determined by plaque assay.
As an additional control, the anti-Fas antibody was pre-incubated with recombinant soluble human Fas (PharMingen, San Diego, CA), 0.5 μg/ml, for 1 h at room temperature prior to use as the primary antibody to stain the specimens.
Where indicated recombinant NFATp proteins were pre-incubated with promoter DNA for five min at 30°C prior to the addition of the remaining general transcription factors (TFIIA, TFIIB, TFIID, TFIIE, TFIIF, and TFIIH) and RNA polymerase II.
The number of plaques was then determined and activity calculated based on the reduction in average number of plaques, in wells where the compound was pre-incubated with the virus, compared to the control wells inoculated with untreated virus.
Purified TOP1 was pre-incubated with GST or GST-BTBD2 for 20 min before addition of the DNA substrate, which was either an end-labeled 36 bp fragment containing a single strong TOP1 cleavage site [ 24 ] , or a 161 bp PvuII-HindIII fragment of pBluescript (Stratagene) [ 25 ] ). End labeling of the DNA substrates was as previously described [ 24 25 ] . Cleavage reactions were continued for 20 min before stopping with 0.5% SDS.