Words similar to pre-incubated

Example sentences for: pre-incubated

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Purified TOP1 was pre-incubated with GST or GST-BTBD2 for 20 min before addition of the DNA substrate, which was either an end-labeled 36 bp fragment containing a single strong TOP1 cleavage site [ 24 ] , or a 161 bp PvuII-HindIII fragment of pBluescript (Stratagene) [ 25 ] ). End labeling of the DNA substrates was as previously described [ 24 25 ] . Cleavage reactions were continued for 20 min before stopping with 0.5% SDS.
For re-immunoprecipitation experiments, associated proteins were eluted from immune complexes in 1% Triton X100 in PBS, then pre-incubated with protein A-sepharose-4CL.
As an additional control, the anti-Fas antibody was pre-incubated with recombinant soluble human Fas (PharMingen, San Diego, CA), 0.5 μg/ml, for 1 h at room temperature prior to use as the primary antibody to stain the specimens.
When I-2 was used, it was pre-incubated with PfPP1 at 32°C for 30 min.
The number of plaques was then determined and activity calculated based on the reduction in average number of plaques, in wells where the compound was pre-incubated with the virus, compared to the control wells inoculated with untreated virus.