Example sentences for: pre-incubated

How can you use “pre-incubated” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • These compounds were pre-incubated with the virus and then the mixture was inoculated on CV-1 cells and residual virus titers were determined by plaque assay.

  • Where indicated recombinant NFATp proteins were pre-incubated with promoter DNA for five min at 30°C prior to the addition of the remaining general transcription factors (TFIIA, TFIIB, TFIID, TFIIE, TFIIF, and TFIIH) and RNA polymerase II.

  • For re-immunoprecipitation experiments, associated proteins were eluted from immune complexes in 1% Triton X100 in PBS, then pre-incubated with protein A-sepharose-4CL.

  • Staining was inhibited when the anti-Fas antibody was pre-incubated with recombinant soluble Fas (Fig.

  • Purified TOP1 was pre-incubated with GST or GST-BTBD2 for 20 min before addition of the DNA substrate, which was either an end-labeled 36 bp fragment containing a single strong TOP1 cleavage site [ 24 ] , or a 161 bp PvuII-HindIII fragment of pBluescript (Stratagene) [ 25 ] ). End labeling of the DNA substrates was as previously described [ 24 25 ] . Cleavage reactions were continued for 20 min before stopping with 0.5% SDS.


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