Example sentences for: pre-hybridized

How can you use “pre-hybridized” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The membrane was pre-hybridized with PERFECTHYB PLUS solution (Sigma) at 68°C for 1 hr, and a cDNA probe for PPT1 was added and incubated at 42°C for overnight.

  • Briefly, filters were pre-hybridized at 65°C in hybridization buffer (5x SSC, 50 mM NaPO 4 , pH 7.4, 2.5x Denhardt's Solution) with 100 μg/ml denatured salmon sperm DNA for two hours.

  • Microarrays were pre-hybridized for 2 hours at 42°C with 5 ml of MicroHyb hybridisation solution (ResGen) plus 5 μg denatured Cot-1 DNA (ResGen) and 5 μg of poly dA.

  • The blots were pre-hybridized in a solution containing 6-strength SSC (150 mM NaCl, 15 mM Na-citrate, pH 7.0), 0.5% SDS, 50% formamide, and 100 mg/ml denatured sperm DNA at 42°C for 2-4 h, and were hybridized overnight in the same buffer containing approximately 10 6cpm/ml of radiolabeled cDNA probe.


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