Words similar to polynucleotide

Example sentences for: polynucleotide

How can you use “polynucleotide” in a sentence? Here are some example sentences to help you improve your vocabulary:

The primers were 5'-labeled using γ- 32P-ATP and T4 polynucleotide kinase according to the standard protocol [ 37 ] . Polymerase reactions were carried out by incubating 2.5 nM template primer with 50 nM of the wild type HIV-1RT or its mutant derivative in a total reaction volume of 6 μL containing 25 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 100 μg/mL bovine serum albumin, 5 mM MgCl
The 5'-end of the sagA/pel mRNA was determined by the extension of the 5'-end 32P-labeled oligonucleotides 5'-ACCTTATTTTAAAAATAAAGTTAA-3' following the method of Sambrook [ 45 ] . Oligonucleotides were labeled with [γ- 32P] ATP (10 mCi/mL in aqueous solution) (Amersham, Arlington Heights, IL) and T4 polynucleotide kinase (Gibco BRL Life Technologies, Rockville, MD).
Genotyping with polymorphic di-, tri-, or tetranucleotide-repeat markers was performed by PCR according to the conditions used in the test PCRs above except that one of the primers was 32P-labeled with T4 polynucleotide kinase.
PCR primer (200 ng) for probe sequence was end-labeled with 32Pγ-ATP and T4 polynucleotide kinase (New England Biolabs, Beverly, MA, USA), and was column purified.
The metal cations direct a 5' nucleoside triphosphate to form a phosphoester bond with the 3' hydroxyl of the preceding nucleotide (the 3'-terminal nucleotide of a growing polynucleotide chain), with the elimination of pyrophosphate [ 6 ] .