Words similar to polylinker

Example sentences for: polylinker

How can you use “polylinker” in a sentence? Here are some example sentences to help you improve your vocabulary:

The TAR vector pARS-SV211-URA was constructed by insertion of the S. cerevisiae URA3 gene between the targeting hooks of pVC-SV211 [ 7 ] and cloning of ARSH4 into the polylinker.
The pVC-SV60-polylinker plasmid contains the same hooks as pVC-SV60 [ 7 ] , but the hooks are separated by approximately 70 bp in the pBluscript polylinker.
The adenovirus (AV) backbone for the aquaporin-1 (AQP1) sense and antisense AV constructs was a replication-deficient "first-generation" AV with deletions of the E1 and E3 genes [ 26 ] . This "empty" AV contains the cytomegalovirus (CMV) promoter and bovine growth hormone polyadenylation (bHG) site separated by a polylinker that was used to clone AQP1 DNA as described previously [ 27 ] . A recombinant AQP1 virus was constructed using a plasmid containing the coding sequence for AQP1, pCHIPev [ 28 ] . pCHIPev was digested and the AQP1 insert was subcloned into the shuttle vector pSKAC, creating pSKAC/AQP1.
The PCR fragments were inserted into a pRS313 polylinker [ 32 ] to generate the basic ARS -containing TAR vector designated as pARS-VN.
Eco RI digestion and complete Bam HI digestion, which cleaves within the polylinker site approximately 700 bp downstream of the rec12 +stop codon in exon 5. The DNA fragment with single-site cleavage by Eco RI at bp position +3, relative to the ATG start codon, was gel purified.