Words similar to polylinker

Example sentences for: polylinker

How can you use “polylinker” in a sentence? Here are some example sentences to help you improve your vocabulary:

The adenovirus (AV) backbone for the aquaporin-1 (AQP1) sense and antisense AV constructs was a replication-deficient "first-generation" AV with deletions of the E1 and E3 genes [ 26 ] . This "empty" AV contains the cytomegalovirus (CMV) promoter and bovine growth hormone polyadenylation (bHG) site separated by a polylinker that was used to clone AQP1 DNA as described previously [ 27 ] . A recombinant AQP1 virus was constructed using a plasmid containing the coding sequence for AQP1, pCHIPev [ 28 ] . pCHIPev was digested and the AQP1 insert was subcloned into the shuttle vector pSKAC, creating pSKAC/AQP1.
The pVC-SV60-polylinker plasmid contains the same hooks as pVC-SV60 [ 7 ] , but the hooks are separated by approximately 70 bp in the pBluscript polylinker.
Eco RI digestion and complete Bam HI digestion, which cleaves within the polylinker site approximately 700 bp downstream of the rec12 +stop codon in exon 5. The DNA fragment with single-site cleavage by Eco RI at bp position +3, relative to the ATG start codon, was gel purified.
The PCR fragments were inserted into a pRS313 polylinker [ 32 ] to generate the basic ARS -containing TAR vector designated as pARS-VN.
The TAR vector pARS-SV211-URA was constructed by insertion of the S. cerevisiae URA3 gene between the targeting hooks of pVC-SV211 [ 7 ] and cloning of ARSH4 into the polylinker.