Words similar to polyclonal
Example sentences for: polyclonal
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Recently, a subset of CD4 +T cells was identified that is present on 5-10% of CD4 +T cells in normal naïve mice and expresses CD25 (the α-chain of IL-2 receptor) [ 4 5 ] . Functional analysis of murine CD4 +CD25 +T cells showed that those cells, which constitutively express cytotoxic T-lymphocyte antigen (CTLA)-4 [ 6 7 8 ] , fail to proliferate or secret cytokines in response to polyclonal or antigen-specific stimulation, but inhibit the activation of conventional responsive T cells [ 1 2 3 8 9 ] . The suppressive activity of the CD4 +CD25 +T cells depends on signaling via the negative regulator of T-cell activation CTLA-4 [ 7 ] and requires a cell-cell interaction that possibly involves cell surface bound transforming growth factor (TGF)-β 1 [ 1 10 ] . It has been shown that B7/CD28 costimulation is essential for the development and homeostasis of the CD4 +CD25 +regulatory T cells [ 6 ] , which play critical roles not only in preventing autoimmunity but also in controlling tumor immunity and transplantation tolerance [ 2 11 ] .
Polyclonal antibodies against a N-terminal fragment of MmCOP1 (amino acid 71-270) were generated in rabbits and affinity purified.
The immunoglobulin V H gene fingerprinting approach [ 23] takes advantage of the wide range of HCDR3 lengths that can occur in human B cells (approximately 5-35 amino acids) to provide an estimate of clonal diversity in polyclonal populations.
Results from Western blot analyses with polyclonal antibodies NMT-4 and NMT-5 showed that nmt55/p54 nrbexpression was absent, or greatly reduced, in most of ER- tumors.
Immunohistochemistry was performed using antibodies to ACTH (rabbit polyclonal anti-ACTH; Chemicon International) or glial fibrillary acid protein (rabbit polyclonal anti-GFAP; Chemicon International).
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