Words similar to polyclonal
Example sentences for: polyclonal
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Additional primary antibodies used were zn-8 to detect Dmgrasp and zrf-1 (Developmental Studies Hybridoma Bank) and polyclonal rabbit anti Pax-2 (Berkeley Antibody Co.).
For IP control, 0.5 μg of normal rabbit IgG was used instead of anti-Cdk2 rabbit polyclonal antibody.
Antibodies: SKP2 (Zymed 32-3300, 1:250 for immunoblotting), SKP2 (Santa Cruz sc-7164, 1:500 for immunofluorescence staining), p27 (Transduction Laboratories K25030, 1:5000 for immunoblotting, 1:1000 for immunofluorescence staining), cyclin A (Neomarkers Ab-6, MS-1061, 1:100 for immunoblotting), cyclin E (Neomarkers Ab-1, RB-012, 2 ug per immunoprecipitation) cyclin E (Neomarkers Ab-2, MS-870, 1:200 for immunoblotting), HRT1 (affinity-purified rabbit polyclonal, gift from R. Deshaies, 1:1000 for immunoblotting), CUL1 (Zymed 71-8700, 1:250 for immunoblotting), SKP1 (Transduction laboratories 610530, 1:5000 for immunoblotting), JAB1/CSN5 (GeneTex MS-Jab11-PX1, 1:1000 for immunoblotting), RB1 (Pharmingen 554136, 1:500 for immunoblotting), CDK2 (Santo Cruz Biotechnology sc-163, 2 ug per immunoprecipitation, 1:500 for immunoblotting), Tubulin (Sigma T5168, 1:2000 for immunoblotting).
Polyclonal antibodies were developed against these peptides; polyclonal antibody NMT-4 recognized a sequence in the amino terminal region of nmt55/p54 nrband polyclonal antibody NMT-5 recognized a sequence in a mid-region of nmt55/p54 nrb(Figure 1B).
To determine if the Pten protein was produced in Wnt-1 TG, Pten +/- tumors that did not undergo LOH, sections were analyzed using an immunohistochemical assay with a polyclonal rabbit antibody.
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