Example sentences for: polybrene

How can you use “polybrene” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Cells were grown to approximately 75% confluence and incubated in the presence of 8 μg/ml polybrene with 2 ml supernatant containing amphotropic retrovirus DFG-IRAP-zeo rcontaining the human IL-1Ra and Streptoalloteichus hindustanus bleomycin-resistance ( Sh ble ; zeo r) genes.

  • Supernatant, generated from transfected GPE86 cells [ 31], was then used to infect the amphotropic packaging cell line PA317 [ 31] in the presence of 4 μg/ml polybrene (Abbott Laboratories, Abbott Park, IL, USA).

  • Both H9 and H9/Tat cells were infected in the presence of 10 ug of polybrene.

  • MSCV/syk expression vectors were packaged into viral particles by transient transfection of 1 × 10 6BOSC cells with 15 μg of plasmid DNA/CaPO 4 precipitates [ 61 ] . BCL 1 .3B3 cells were infected with 500 μl of a 1:2 dilution of viral supernatant in growth medium containing 4 μg/ml polybrene (Sigma-Aldrich, Inc.).


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