Words similar to podocytes
Example sentences for: podocytes
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Murine podocytes immortalized with interferon-inducible temperature-sensitive SV-40 T antigen ( ts -SV-40 T) were isolated and infected with replication incompetent GFP-expressing HIV-1 virus, replication incompetent GFP-expressing single-gene mutants of HIV-1, or replication incompetent GFP-expressing control lentivirus containing GFP but no HIV-1 genes as previously described [ 7 8 ] . In all cases, 70% to 80% of podocytes showed GFP expression 4 days post infection (data not shown).
In contrast, cell-cell contact in wild-type and control-infected, but not HIV-1-infected podocytes, caused a severe down-regulation of cyclin D 1 transcript and protein concomitant with the up-regulation of synaptopodin, a podocyte differentiation marker [ 7 28 ] , by the end of the 14-day differentiation period.
In experiments utilizing the single gene mutant of HIV-1, total RNA was extract from infected podocytes 14 days after switching confluent cultures to 37°C.
Total RNA and protein were extracted from wild-type, control-infected, and HIV-1 infected podocytes during the Reiser, et al.
Infection of cultured podocytes with replication incompetent HIV-1 demonstrated that HIV-1 gene expression leads to the proliferation and dedifferentiation of the infected podocytes [ 6 7 8 ] , recapitulating the phenotypes observed in HIVAN specimens [ 9 10 ] . Similarly, renal expression of replication incompetent HIV-1 proviral transgenes in mice and rats produces a proliferative renal lesion that is indistiguisable both histopathologically and clinically from human HIVAN [ 11 12 13 14 ] . These observations raise the possibility that HIV-1 gene products may interact with mitogenic pathways in infected renal epithelium to cause aberrant cell-cycle engagement.
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