Words similar to podocytes
Example sentences for: podocytes
How can you use “podocytes” in a sentence? Here are some example sentences to help you improve your vocabulary:
After the 14-day podocyte differentiation protocol described above, total RNA was extracted from wild-type and HIV-1-infected podocytes before treatment with flavopiridol (Developmental Therapeutics Program, National Cancer Institute) (day 14), two days after suppression of HIV-1 transcription with media containing 50 nM flavopiridol (day 16), and one day after drug washout using media without flavopiridol (day 17).
Analysis of cyclin D 1 transcript levels in podocytes infected with HIV-1 singly mutated with premature stop codons in the genes encoding the various structural and regulatory proteins of HIV-1 (except tat , which is present in every virus) showed that nef -deficient virus did not significantly up-regulate cyclin D 1 expression when compared to the other mutant viruses (Figure 5).
The ongoing proliferation of HIV-1-infected podocytes following cell-cell contact led to the formation of multiple foci (Figure 2C) with intense, heterogenous nuclear cyclin D 1 immunofluorescence consistent with ongoing asynchronous proliferation in this population of cells (Figure 3).
Moreover, HIV-1-infected podocytes, but not control-infected podocytes, had readily detectable phospho-pRb (Ser780) at the end of the differentiation period, suggesting that the stably up-regulated cyclin D 1 protein remained active in promoting cell-cycle progression (Figure 2B).
In contrast, cell-cell contact in wild-type and control-infected, but not HIV-1-infected podocytes, caused a severe down-regulation of cyclin D 1 transcript and protein concomitant with the up-regulation of synaptopodin, a podocyte differentiation marker [ 7 28 ] , by the end of the 14-day differentiation period.
Loading...