Words similar to podocytes
Example sentences for: podocytes
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In experiments utilizing the single gene mutant of HIV-1, total RNA was extract from infected podocytes 14 days after switching confluent cultures to 37°C.
Moreover, HIV-1-infected podocytes, but not control-infected podocytes, had readily detectable phospho-pRb (Ser780) at the end of the differentiation period, suggesting that the stably up-regulated cyclin D 1 protein remained active in promoting cell-cycle progression (Figure 2B).
These data indicate that HIV-1 expression in infected podocytes induces cyclin D 1 and phospho-pRb (Ser780) expression, hallmarks of cyclin D 1 -mediated G 1 → S progression.
protocol to study subconfluent podocyte growth and podocyte growth during establishment of mature cell-cell contacts between differentiated podocytes [ 27 28 ] . Briefly, RNA and protein were extracted from parallel plates of podocytes using TRIZOL Reagent or RIPA buffer, respectively, under three conditions: 1) during sub-confluent growth at 33°C; 2) immediately upon reaching confluency (day zero); and 3) on day 14 after switching confluent cultures to 37°C.
Murine podocytes immortalized with interferon-inducible temperature-sensitive SV-40 T antigen ( ts -SV-40 T) were isolated and infected with replication incompetent GFP-expressing HIV-1 virus, replication incompetent GFP-expressing single-gene mutants of HIV-1, or replication incompetent GFP-expressing control lentivirus containing GFP but no HIV-1 genes as previously described [ 7 8 ] . In all cases, 70% to 80% of podocytes showed GFP expression 4 days post infection (data not shown).
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