Words similar to podocyte

Example sentences for: podocyte

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The following results of this study suggest that HIV-1 expression leads to cyclin D 1 -mediated G 1 → S progression in infected podocytes: cyclin D 1 transcript and protein levels are markedly up-regulated, phospho-Rb (Ser780) levels are increased, cyclin D 1 protein and phospho-Rb (Ser780) levels do not decrease with podocyte cell-cell contact and differentiation, and the up-regulation of cyclin D 1 requires the expression of HIV-1 genes, particularly HIV-1 nef.
HIV-1 expression in cultured podocytes recapitulates the phenotypic abnormalities induced by HIV-1 expression in infected podocytes in vivo, specifically proliferation, loss of contact-mediated growth control, and dedifferentiation [ 6 7 8 ] . To determine if these phenotypes were marked by enhanced cyclin D 1 and phospho-pRb (Ser780) expression, we utilized the podocyte cell-culture protocol developed by Rieser et al.
[ 27 28 ] to study both sub-confluent podocyte growth and podocyte growth during the formation of mature cell-cell contacts at confluence.
Podocyte cell-cell contact
Following the 14 day podocyte differentiation protocol on coverslips to establish mature podocytes, wild-type and HIV-1 infected podocytes underwent immunofluorescent detection of cyclin D 1 as follows: coverslips were washed three times with 1 × PBS, fixed in 60% acetone/3.