Words similar to podocyte
Example sentences for: podocyte
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Cyclin D 1 is characterized as the major D-type cyclin controlling G 1 -phase progression in kidney cells, both during normal and abnormal proliferation [ 22 23 ] . To determine whether cyclin D 1 may be active in promoting G 1 → S progression in kidneys expressing HIV-1 genes, we utilized a well-characterized HIV-1 transgenic mouse model (Tg26) of HIVAN [ 11 12 13 ] . Secondary to renal expression of the HIV-1 NL4-3 Δ gag-pol proviral transgene, adolescent Tg26 transgenics develop a progressive, proliferative renal disease marked by podocyte and tubular epithelial hyperplasia that is indistinguishable both clinically and histopathologically from human HIVAN.
RNA and protein samples were analyzed as follows: Podocyte RNA (10 μg) was analyzed by northern blot using probes for HIV-1 nef-LTR [ 7 ] , mouse cyclin D 1 (cDNA generated by RT-PCR from mouse podocytes and verified by sequence analysis), synaptopodin [ 7 ] , and G3PDH.
protocol to study subconfluent podocyte growth and podocyte growth during establishment of mature cell-cell contacts between differentiated podocytes [ 27 28 ] . Briefly, RNA and protein were extracted from parallel plates of podocytes using TRIZOL Reagent or RIPA buffer, respectively, under three conditions: 1) during sub-confluent growth at 33°C; 2) immediately upon reaching confluency (day zero); and 3) on day 14 after switching confluent cultures to 37°C.
Following the 14 day podocyte differentiation protocol on coverslips to establish mature podocytes, wild-type and HIV-1 infected podocytes underwent immunofluorescent detection of cyclin D 1 as follows: coverslips were washed three times with 1 × PBS, fixed in 60% acetone/3.
Following 7.5% SDS/PAGE, podocyte protein (20 μg) was analyzed by western blot for cyclin D 1 (ECL detection of a 1:1000 dilution of rabbit anti-cyclin D 1 antibody, Santa Cruz BioTechnology).
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