Example sentences for: podocyte

How can you use “podocyte” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • [ 27 28 ] to study both sub-confluent podocyte growth and podocyte growth during the formation of mature cell-cell contacts at confluence.

  • Podocyte cell-cell contact

  • protocol to study subconfluent podocyte growth and podocyte growth during establishment of mature cell-cell contacts between differentiated podocytes [ 27 28 ] . Briefly, RNA and protein were extracted from parallel plates of podocytes using TRIZOL Reagent or RIPA buffer, respectively, under three conditions: 1) during sub-confluent growth at 33°C; 2) immediately upon reaching confluency (day zero); and 3) on day 14 after switching confluent cultures to 37°C.

  • RNA and protein samples were analyzed as follows: Podocyte RNA (10 μg) was analyzed by northern blot using probes for HIV-1 nef-LTR [ 7 ] , mouse cyclin D 1 (cDNA generated by RT-PCR from mouse podocytes and verified by sequence analysis), synaptopodin [ 7 ] , and G3PDH.

  • Following the 14 day podocyte differentiation protocol on coverslips to establish mature podocytes, wild-type and HIV-1 infected podocytes underwent immunofluorescent detection of cyclin D 1 as follows: coverslips were washed three times with 1 × PBS, fixed in 60% acetone/3.


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