Words similar to podocyte
Example sentences for: podocyte
How can you use “podocyte” in a sentence? Here are some example sentences to help you improve your vocabulary:
Cyclin D 1 is characterized as the major D-type cyclin controlling G 1 -phase progression in kidney cells, both during normal and abnormal proliferation [ 22 23 ] . To determine whether cyclin D 1 may be active in promoting G 1 → S progression in kidneys expressing HIV-1 genes, we utilized a well-characterized HIV-1 transgenic mouse model (Tg26) of HIVAN [ 11 12 13 ] . Secondary to renal expression of the HIV-1 NL4-3 Δ gag-pol proviral transgene, adolescent Tg26 transgenics develop a progressive, proliferative renal disease marked by podocyte and tubular epithelial hyperplasia that is indistinguishable both clinically and histopathologically from human HIVAN.
[ 27 28 ] to study both sub-confluent podocyte growth and podocyte growth during the formation of mature cell-cell contacts at confluence.
Following 7.5% SDS/PAGE, podocyte protein (20 μg) was analyzed by western blot for cyclin D 1 (ECL detection of a 1:1000 dilution of rabbit anti-cyclin D 1 antibody, Santa Cruz BioTechnology).
protocol to study subconfluent podocyte growth and podocyte growth during establishment of mature cell-cell contacts between differentiated podocytes [ 27 28 ] . Briefly, RNA and protein were extracted from parallel plates of podocytes using TRIZOL Reagent or RIPA buffer, respectively, under three conditions: 1) during sub-confluent growth at 33°C; 2) immediately upon reaching confluency (day zero); and 3) on day 14 after switching confluent cultures to 37°C.
After the 14-day podocyte differentiation protocol described above, total RNA was extracted from wild-type and HIV-1-infected podocytes before treatment with flavopiridol (Developmental Therapeutics Program, National Cancer Institute) (day 14), two days after suppression of HIV-1 transcription with media containing 50 nM flavopiridol (day 16), and one day after drug washout using media without flavopiridol (day 17).
Loading...