Words similar to pmsf

Example sentences for: pmsf

How can you use “pmsf” in a sentence? Here are some example sentences to help you improve your vocabulary:

Cell lysates for ubiquitylation assays were prepared by hypotonic lysis on ice in 20 mM Hepes pH 7.5, 15 mM MgCl 2 , 5 mM KCl, 1 mM DTT, 10 μg/ml leupeptin, 10 μg/ml pepstatin, 1 mM PMSF, 17 μg/ml aprotinin as described previously [ 30 ] . Lysates were cleared by centrifugation and used without prior freezing.
Cell extracts were prepared using a lysis buffer consisting of 50 mM HEPES, pH 7.5, 150 mM NaCl, 1 mM EDTA, 2.5 mM EGTA, 10% glycerol, 0.1% Tween-20, 1 mM DTT, 1 mM NaF, 0.1 mM Na 3 VO 4 , 10 μg/ml leupeptin, 2 μg/ml Aprotinin, and 0.1 mM PMSF.
Cells were lysed in a buffer containing 50 mM Tris-HCl (pH 7.6), 5 mM EDTA, 150 mM NaCl, 0.5 % NP-40, and 0.5% Triton X-100 containing 1 μg/ml leupeptin, aprotinin and antipain; 1 mM sodiumorthovanadate; and 0.5 mM PMSF (all reagents were obtained from Sigma Chemical Co.).
5 mM Tris-HCl, 10% glycerol, 1% SDS, pH 6.8) containing protease inhibitor (1 mM PMSF) at 4°C.
Nuclei were prepared from HeLa cells by standard procedures [ 35] and resuspended in 10 packed nuclei volumes of buffer A (10 mM Hepes (pH 7.9) 1.5 mM MgCl 2 , 10 mM KCl, 0.5 mM DTT, and 0.5 mM PMSF).