Words similar to pmsf

Example sentences for: pmsf

How can you use “pmsf” in a sentence? Here are some example sentences to help you improve your vocabulary:

In order to observe changes at the onset of apoptosis, only adherent cell populations were trypsinized, pelleted 5 minutes at 500 × g, and lysed in ice cold lysis buffer (10 mM Tris-HCl (pH 7.5), 1 mM MgCl 2 , 1 mM EGTA, 0.1 mM PMSF, 5 mM β-mercaptoethanol, 0.5% CHAPS, 10% glycerol) for 30 minutes at 4°C.
Cell lysates for immunoprecipitation were prepared in IP-LB (25 mM Tris, pH 7.4, 150 mM NaCl, 0.5 % Triton X100, 10 μg/ml leupeptin, 10 μg/ml pepstatin, 1 mM PMSF, 17 μg/ml aprotinin).
Cell extracts were prepared using a lysis buffer consisting of 50 mM HEPES, pH 7.5, 150 mM NaCl, 1 mM EDTA, 2.5 mM EGTA, 10% glycerol, 0.1% Tween-20, 1 mM DTT, 1 mM NaF, 0.1 mM Na 3 VO 4 , 10 μg/ml leupeptin, 2 μg/ml Aprotinin, and 0.1 mM PMSF.
5 M NaCI/1 mM DTT/1 mM PMSF/leupeptin/aprotinin/600 μM Na 3 VO 4 /50 mM NaF).
Cells were lysed for 15 min at 4°C in EBC buffer (50 mM Tris-HCl, pH 8.0, 120 mM NaCl, 0.5% Nonidet P-40) containing 10 ug/ml of the protease inhibitors aprotinin, leupeptin, and phenylmethylsulfonylfluoride (PMSF).