Example sentences for: pmsf

How can you use “pmsf” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Each sample was further homogenized by passage through a 21-gauge needle followed by an addition of 10 μl PMSF (stock solution: 50 mM).

  • The Triton-insoluble fractions were then scraped into cold Triton X-100 lysis buffer [50 mM Tris/HCl (pH 7.4); 100 mM NaCl; 50 mM NaF; 5 mM EDTA; 40 mM glycerophosphate; 1 mM sodium orthovanadate; 100 μM PMSF; 1 μM leupeptin; 1 μM pepstatin A; 1% (v/v) Triton X-100] and incubated for 20 min on ice, and clarified by centrifugation at 13000 g for 5 min at 4°C.

  • 5, 250 mM sucrose with protease inhibitors PMSF, Leupeptin, Aprotinin) by a dounce homogenizer times10 strokes.

  • Nuclei were prepared from HeLa cells by standard procedures [ 35] and resuspended in 10 packed nuclei volumes of buffer A (10 mM Hepes (pH 7.9) 1.5 mM MgCl 2 , 10 mM KCl, 0.5 mM DTT, and 0.5 mM PMSF).

  • 4 M sorbitol, 40 mM HEPES (pH = 7.2), 0.5 mM MgCl 2 ). Cells were resuspended in buffer S, 1 mM PMSF, 10 mM β-mercaptoethanol and incubated for 10 min at 30°C.

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