Words similar to pmsf

Example sentences for: pmsf

How can you use “pmsf” in a sentence? Here are some example sentences to help you improve your vocabulary:

The nuclei prepared above were suspended in 10 ml of ice-cold TP buffer (10 mM Tris/HCl, pH 8.0, 10 mM Na 2 HPO 4 , 1 mM PMSF) containing 0.3 mg/ml heparin and 36 u/ml of DNase I. The suspension was gently stirred on a magnetic stirrer for 60 minutes at 4°C and 15 minutes at room temperature.
Protease inhibitors are 40 μg/ml bestatin, 0.7 μg/ml pepstatin A, 1 mM PMSF, 50 μM leupeptin, 1 mM benzamidine, and 1 mM EGTA.
Briefly, gelatin-conjugated sepharose bead columns were pre-washed with PBS containing 2 mM EDTA and 0.1 mM PMSF.
A549 cells or NHBEs were treated with CSC as described above and whole-cell protein extracts were prepared by scraping cells into lysis buffer containing 20 mM Tris-HCl (pH 8.0), 1 mM EDTA, 1 mM sodium orthovanadate, 1 mM sodium fluoride, 1 μg/ml each of leupeptin and pepstatin, and 1 μM PMSF.
Cell lysates for ubiquitylation assays were prepared by hypotonic lysis on ice in 20 mM Hepes pH 7.5, 15 mM MgCl 2 , 5 mM KCl, 1 mM DTT, 10 μg/ml leupeptin, 10 μg/ml pepstatin, 1 mM PMSF, 17 μg/ml aprotinin as described previously [ 30 ] . Lysates were cleared by centrifugation and used without prior freezing.