Words similar to pmsf

Example sentences for: pmsf

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RAW cells expressing IDO and vector only controls were harvested in cell lysis buffer (PBS, 1%NP40, 0.5% sodium deoxycholate, 0.1% SDS, 150 ng/ml PMSF, 100 ng/ml aprotinin) and 25 μg of cell protein was electrophoresed on 10% polyacrylamide gels overlayed with a 5% stacking gel.
5 mM Tris-HCl, 10% glycerol, 1% SDS, pH 6.8) containing protease inhibitor (1 mM PMSF) at 4°C.
For immunoprecipitations, labeled cellular proteins were extracted with lysis buffer (1% of the indicated detergent, 20 mM Hepes, pH 7.4, 150 mM NaCl, 2 mM PMSF, 10 μg/ml leupeptin, 10 μg/ml aprotinin, 2 mM MgCl2, 0.5 mM CaCl2) for 60 minutes at 4°C.
Cells were lysed in lysis buffer (20 mM HEPES, pH 7.4, containing 100 mM NaCl, 5 mM MgCl 2 , 5 mM EDTA, 0.5% Triton X-100 and 1 mM PMSF) with 1 × proteasome inhibitor cocktail (Boehringer Mannhein).
The cell pellets were suspended in 150-300 μl ice-cold RIPA lysis buffer (1% NP-40, 0.5% Na-deoxycholate, 0.1% SDS in PBS, 0.5 mM Phenylmethylsulfonyl fluoride (PMSF), 0.02 mg/ml Aprotinin, 1 mM Na 3 VO 4 ) and incubated for 30 min on ice.