Words similar to pmsf

Example sentences for: pmsf

How can you use “pmsf” in a sentence? Here are some example sentences to help you improve your vocabulary:

Nuclei were prepared from HeLa cells by standard procedures [ 35] and resuspended in 10 packed nuclei volumes of buffer A (10 mM Hepes (pH 7.9) 1.5 mM MgCl 2 , 10 mM KCl, 0.5 mM DTT, and 0.5 mM PMSF).
5 M NaCI/1 mM DTT/1 mM PMSF/leupeptin/aprotinin/600 μM Na 3 VO 4 /50 mM NaF).
In order to observe changes at the onset of apoptosis, only adherent cell populations were trypsinized, pelleted 5 minutes at 500 × g, and lysed in ice cold lysis buffer (10 mM Tris-HCl (pH 7.5), 1 mM MgCl 2 , 1 mM EGTA, 0.1 mM PMSF, 5 mM β-mercaptoethanol, 0.5% CHAPS, 10% glycerol) for 30 minutes at 4°C.
137 M NaCl, 2% Nonidet P-40, 10% SDS, 1% sodium deoxycholate, 20 mM Tris, pH 8.0, 2 mM PMSF, 10 μl/ml protease inhibitor cocktail), which solubilizes cytoskeletal and nuclear matrices.
Next, the cells were resuspended at 3 × 10 6cells/ml in ice cold sonication buffer (250 mM sucrose; 10 mM HEPES, pH 7.4; 1 mM EDTA; 5 mM MgCl 2 ; 1 mM DTT; 0.1 mM GTP; 1 μg/ml leupeptin, 1 μg/ml pepstatin A, and 1 μg/ml aprotinin; 1 mM PMSF) and sonicated for 10 seconds.