Example sentences for: pmsf
How can you use “pmsf” in a sentence? Here are some example sentences to help you improve your vocabulary:
Cell lysates for immunoprecipitation were prepared by disrupting cells in immunoprecipitation buffer (20 mM Tris/HCl, pH 7.4; 150 mM NaCl; 0.5 % Triton X-100, 10 μg/ml leupeptin, 10 μg/ml pepstatin, 5 μg/ml aprotinin, 1 mM PMSF).
5, 250 mM sucrose with protease inhibitors PMSF, Leupeptin, Aprotinin) by a dounce homogenizer times10 strokes.
Cell lysates for immunoprecipitation were prepared in IP-LB (25 mM Tris, pH 7.4, 150 mM NaCl, 0.5 % Triton X100, 10 μg/ml leupeptin, 10 μg/ml pepstatin, 1 mM PMSF, 17 μg/ml aprotinin).
After washing, plasma containing 2 mM EDTA and 0.1 mM PMSF was passed over the column twice, then washed with at least 4 volumes of PBS/EDTA/PMSF.
Phenylmethylsulfonylfluoride (PMSF) was purchased from Sigma.
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