Words similar to pmet
Example sentences for: pmet
How can you use “pmet” in a sentence? Here are some example sentences to help you improve your vocabulary:
To confirm that these genomic fragments indeed carried the TAB genes, complementing fragments were cloned into the integrating vector [pRS305, LEU2], and linearized to target integration into the tab locus in a cdc15Δ::TRP1 tab [pMET3-CDC15, URA3 ] strain.
To determine if Tab +phenotypes were due to single mutations, cdc15Δ tab strains were crossed to cdc15Δ [pMET3-cdc15-2, URA3] and the resulting diploids were sporulated and dissected.
All cdc15Δ tab mutants were also crossed to strains harboring either [pMET3-CDC15, URA3] [pRS313, HIS3] or [pMET3-CDC15, URA3] [pRS315, LEU2] to introduce these plasmids.
The transformants lost their Tab +phenotype, and when crossed to cdc15Δ::TRP1 [pMET3-CDC15, URA3], the diploid yielded no spores that could bypass cdc15Δ (n = 40-50).
The resulting plasmids were transformed into cdc15Δ::TRP1 tab [pMET3-CDC15, URA3 ] to identify the ORF that reversed the Tab +phenotype.