Words similar to pmet
Example sentences for: pmet
How can you use “pmet” in a sentence? Here are some example sentences to help you improve your vocabulary:
To confirm that these genomic fragments indeed carried the TAB genes, complementing fragments were cloned into the integrating vector [pRS305, LEU2], and linearized to target integration into the tab locus in a cdc15Δ::TRP1 tab [pMET3-CDC15, URA3 ] strain.
To assay if SIC 1 or HCT1 was required for the Tab +phenotype of tab mutants, SIC1 or HCT1 was replaced by his5 +in cdc15Δ::TRP1 tab [pMET3-CDC15, URA3 ] strains.
The scheme is outlined in Figure 2. Ninety independent cultures of cdc15Δ [pMET3-cdc15-2, URA3 ] (WY221) were grown at 25°C in synthetic minimal medium + 2% glucose (SD) in the absence of methionine.
PCR analysis confirmed that all twenty five colonies had lost the [ pMET3-cdc15-2, URA3 ] plasmid, indicating that they were true bypassers of cdc15Δ.
The transformants lost their Tab +phenotype, and when crossed to cdc15Δ::TRP1 [pMET3-CDC15, URA3], the diploid yielded no spores that could bypass cdc15Δ (n = 40-50).