Words similar to pmet

Example sentences for: pmet

How can you use “pmet” in a sentence? Here are some example sentences to help you improve your vocabulary:

To assay if SIC 1 or HCT1 was required for the Tab +phenotype of tab mutants, SIC1 or HCT1 was replaced by his5 +in cdc15Δ::TRP1 tab [pMET3-CDC15, URA3 ] strains.
To assign tab mutants to linkage groups, cdc15Δ tab [pMET3-CDC15, URA3 ] strains were crossed against each other, and the diploids were sporulated and dissected.
The resulting plasmids were transformed into cdc15Δ::TRP1 tab [pMET3-CDC15, URA3 ] to identify the ORF that reversed the Tab +phenotype.
To introduce tab mutations into the wild type background, cdc15Δ tab strains were crossed to CDC15 TAB, and tab spores were verified by crossing to cdc15Δ [pMET3-CDC15, URA3], and demonstrating that half of the resulting cdc15Δ segregants were 5-FOA resistant.
To construct [ pMET3-CDC15, URA3 ] (pWS100) and [ pMET3-cdc15-2, URA3 ] (pWS109), the corresponding genes (from start codon to 300 bps downstream of the stop codon) were amplified by PCR from genomic DNA of wild type (RJD381) or cdc15-2 (RJD619) cells, and cloned into the SpeI and SacII sites of RDB620 (a derivative of pRS316 containing the MET3 promoter inserted between the HindIII and EcoRI sites).