Words similar to pmet

Example sentences for: pmet

How can you use “pmet” in a sentence? Here are some example sentences to help you improve your vocabulary:

The transformants lost their Tab +phenotype, and when crossed to cdc15Δ::TRP1 [pMET3-CDC15, URA3], the diploid yielded no spores that could bypass cdc15Δ (n = 40-50).
PCR analysis confirmed that all twenty five colonies had lost the [ pMET3-cdc15-2, URA3 ] plasmid, indicating that they were true bypassers of cdc15Δ.
To construct [ pMET3-CDC15, URA3 ] (pWS100) and [ pMET3-cdc15-2, URA3 ] (pWS109), the corresponding genes (from start codon to 300 bps downstream of the stop codon) were amplified by PCR from genomic DNA of wild type (RJD381) or cdc15-2 (RJD619) cells, and cloned into the SpeI and SacII sites of RDB620 (a derivative of pRS316 containing the MET3 promoter inserted between the HindIII and EcoRI sites).
To introduce tab mutations into the wild type background, cdc15Δ tab strains were crossed to CDC15 TAB, and tab spores were verified by crossing to cdc15Δ [pMET3-CDC15, URA3], and demonstrating that half of the resulting cdc15Δ segregants were 5-FOA resistant.
To determine if Tab +phenotypes were due to single mutations, cdc15Δ tab strains were crossed to cdc15Δ [pMET3-cdc15-2, URA3] and the resulting diploids were sporulated and dissected.