Words similar to pmet
Example sentences for: pmet
How can you use “pmet” in a sentence? Here are some example sentences to help you improve your vocabulary:
The resulting plasmids were transformed into cdc15Δ::TRP1 tab [pMET3-CDC15, URA3 ] to identify the ORF that reversed the Tab +phenotype.
To determine if Tab +phenotypes were due to single mutations, cdc15Δ tab strains were crossed to cdc15Δ [pMET3-cdc15-2, URA3] and the resulting diploids were sporulated and dissected.
The scheme is outlined in Figure 2. Ninety independent cultures of cdc15Δ [pMET3-cdc15-2, URA3 ] (WY221) were grown at 25°C in synthetic minimal medium + 2% glucose (SD) in the absence of methionine.
To introduce tab mutations into the wild type background, cdc15Δ tab strains were crossed to CDC15 TAB, and tab spores were verified by crossing to cdc15Δ [pMET3-CDC15, URA3], and demonstrating that half of the resulting cdc15Δ segregants were 5-FOA resistant.
All cdc15Δ tab mutants were also crossed to strains harboring either [pMET3-CDC15, URA3] [pRS313, HIS3] or [pMET3-CDC15, URA3] [pRS315, LEU2] to introduce these plasmids.