Example sentences for: pmet

How can you use “pmet” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The resulting plasmids were transformed into cdc15Δ::TRP1 tab [pMET3-CDC15, URA3 ] to identify the ORF that reversed the Tab +phenotype.

  • To determine if Tab +phenotypes were due to single mutations, cdc15Δ tab strains were crossed to cdc15Δ [pMET3-cdc15-2, URA3] and the resulting diploids were sporulated and dissected.

  • The scheme is outlined in Figure 2. Ninety independent cultures of cdc15Δ [pMET3-cdc15-2, URA3 ] (WY221) were grown at 25°C in synthetic minimal medium + 2% glucose (SD) in the absence of methionine.

  • To introduce tab mutations into the wild type background, cdc15Δ tab strains were crossed to CDC15 TAB, and tab spores were verified by crossing to cdc15Δ [pMET3-CDC15, URA3], and demonstrating that half of the resulting cdc15Δ segregants were 5-FOA resistant.

  • All cdc15Δ tab mutants were also crossed to strains harboring either [pMET3-CDC15, URA3] [pRS313, HIS3] or [pMET3-CDC15, URA3] [pRS315, LEU2] to introduce these plasmids.


How many words do you know? Try our free vocabulary size test!


Search

Search for example sentences

Loading Loading...
Quantcast