Words similar to plexa

Example sentences for: plexa

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pVT725 was created by replacing the ampicillin resistance cassette on pLexA (Clontech) with a kanamycin resistance gene (obtained from pGBKT7 (Clontech) by PCR) via homologous recombination in yeast.
The CAS peptide was transferred to pLexA as follows: Two complementary oligos which encoded the 11 amino acid CAS peptide (oVT2899: AATTCTGGAGCTTCTGGATCCAAGAATGGAATCAAAGTTAAG, and oVT2900: GGCCGCTTAACTTTGATTCCATTCTTGGATCCAGAAGCTCCAG) were annealed in PCR buffer and cloned using standard methods into pVT725 via EcoRI and NotI restriction sites.