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Example sentences for: placz

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The plasmid pLacZ contains the LacZ coding region from pSVβgal subcloned into the HindIII / EcoRI site of pUC19.
Three pUC-based plasmids were examined for the potential to form S1 nuclease sensitive structures: pTK1 which contains a 3.6 Kb HSV-1 DNA fragment including the tk coding region, pTK2 containing a 3.7 Kb HSV-2 DNA fragment including the tk coding region, and pLacZ which contains the 3.4 Kb LacZ coding region.
The transfection efficiency was normalized by determining β-galactosidase activity in the lysates from the cotransfected pLacZ plasmid.
Figure 2shows that treatment of pTK1 and pLacZ with S1 nuclease did not alter the restriction enzyme profile, suggesting that in this assay, the formation of S1 nuclease-sensitive structures was below the level of detection (Fig.