Words similar to plating
Example sentences for: plating
How can you use “plating” in a sentence? Here are some example sentences to help you improve your vocabulary:
Therefore, neurons were preincubated with nerve growth factor (NGF; 200 ng/ml) for 21 hours prior to plating on sections of adult forebrain.
Mutagenesis of SWY2089 and SWY2090 with ethylmethane sulfonate (EMS; Sigma, St. Louis, MO) was performed as described with the following modifications [ 67 ] . First, cells were mutagenized for 60, 75, and 90 minutes, followed by plating a fraction of the culture to determine cell viability at 23°.
Mesenchymal differentiation was induced by plating 10 × 10 3 to 25 × 10 3 cells/cm 2 on a monolayer of OP9 cells in the presence of 20% heat-inactivated FBS in alpha MEM medium.
Cells were harvested by centrifugation (800 rpm in a clinical centrifuge) and were grown and serially passaged as previously described [ 36 ] . For experiments, cells from passages four to six were used with an initial plating density of 10-20 cells/mm 2. When added, ascorbic acid and TGFβ1 (R&D Systems) were used at 50μg/ml and 10 ng/ml, respectively.
Transient transfections of β-catenin, β-galactosidase and Wnt reporter plasmids were performed using the Fugene reagent 24 hours after initial plating (Roche Molecular Biochemicals).
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