Words similar to plated
Example sentences for: plated
How can you use “plated” in a sentence? Here are some example sentences to help you improve your vocabulary:
Microglia were characterized as described earlier [ 7 ] . Depending on the particular experimental protocol (see below), microglia averaging > than 95% viability were plated in either 60 mm × 15 mm polystyrene cell culture dishes, 96-well cell culture clusters, or 24-well cell culture clusters, with DMEM supplemented with 10% FBS + 120 U/ml P + 12 μg/ml S, and placed in a humidified 5% CO 2 incubator at 36°C 18-24 hours prior to the experiments.
1 × 10 6cells in 100 μl 199 medium containing 2% serum were plated on the underside of transwell membranes by inverting the insert.
Cells were plated onto glass coverslips in 6 well tissue culture plates in selective medium, induced the next day with 1 μg/ml Tet and fixed with 2% paraformaldehyde 24 hours later.
Mononucleated cells were resuspended in plating medium containing 78% Dulbecco's modified Eagle's medium (DMEM) with low glucose (Life Technologies, Rockville, MD), 10 % horse serum (HS, Sigma, St. Louis, MO), 10 % fetal bovine serum (FBS, Sigma, St. Louis, MO), 2% chicken embryo extract (CEE, Life Technologies, Rockville, MD) and plated on plastic culture dishes coated with gelatin at a density of ∼ 1 × 10 4cells per dish.
G418-resistant colonies from BRCA1 antisense infected BG-1 cells and control vector LXSN-infected BG-1 cells were pooled, pretreated for 5 days in phenol red-free, DMEM/F-12 medium (Gibco/Life Technologies) supplemented with 10% charcoal/dextran treated serum (Hyclone, Logan, UT, USA), then plated at 2.5 × 10 6cells per 100mm dish in triplicate for growth curve analysis in the absence or presence of estrogen (10 -8mol/l; 17β-Estradiol; 1,3,5 (10) - Estratriene 3, 17β-diol; Sigma, St Louis, MO, USA).
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