Example sentences for: plated

How can you use “plated” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • FLAG epitope tagged wild-type and S37F mutant β-catenin in pCINeo expression vectors were the kind gifts of Dr. Patrice Morin [ 26 ] . Cells were plated at a density of 300,000 per 60 mm plate.

  • For immunofluorescence staining, LNCaP cells were plated onto poly-lysine coated glass coverslips.

  • Briefly, rat neonatal microglia or cerebellar granule cells (60,000 cells / well) were plated in LPS-free 96-well cell culture clusters 24 hours prior to the experiments in either 0.1 ml DMEM supplemented with 10% FBS + 120 U/ml P + 12 μg/ml S (microglia), or 0.1 ml serum-free Neurobasal A medium with B27 supplement (cerebellar granule neurons).

  • For microscopic observation, cells were plated in chambered coverslips (Nalge Nunc) for 2 hours in fresh medium, washed once with Imaging Buffer (20 mM MES pH 6.8, 25 mM potassium chloride, 2 mM magnesium sulfate, 0.2 mM calcium chloride) and covered with 1.5 ml of the same buffer.

  • RPE cells were then removed under a dissecting microscope, dissociated by trituration using a Pasteur pipette, and then plated on poly-L-lysine (25 mg/liter in water) coated 35-cm 2culture dishes.


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