Words similar to plated
Example sentences for: plated
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The transformation mix was plated onto several LB-AMP plates containing Xgal and isopropyl β-D thiogalactoside (IPTG).
Two-fold serial dilutions of HIV-1 IIIB treated with distinct anionic polymers, and separated from these polymers by precipitation with 3% PEG or by centrifugation at 14,000 rpm for 1 h, and control virus (100 μl), respectively, were added to HeLa-CD4-LTR-β-gal cells which had been plated a day before infection in 96-well plates at 1 × 10 4cells/well in 100 μl of DMEM medium containing 10% fetal bovine serum (FBS).
The cells were resuspended in MEM containing D-valine and 15% dialyzed fetal calf serum and plated on concavalin A-coated round glass coverslips.
RNA was isolated, processed, and used for RT-PCR as described [ 51 52 ] . For polysome analysis, BS153 and Ax4 cells were harvest from bacterial growth plates before noticeable presence of Dictyostelium cell growth . After removal of bacteria, cells were plated for development as described above.
Identification of diploid spore colonies and their haploidization with m -flourophenylalanine ( m -FPA) [ 55 ] were as described [ 38 ] . Fifty haploidized colonies derived from each diploid spore colony were replica plated to differentially supplemented minimal media to genotype the