Words similar to plated
Example sentences for: plated
How can you use “plated” in a sentence? Here are some example sentences to help you improve your vocabulary:
For soft agar assay, clones were plated into 10 60-mm dishes at 1 × 10 5cells/dish containing 0.3% bactopeptone agar with or without added estrogen (10 -8mol/l) in phenol red-free medium with 10% stripped serum in order to test for anchorage independent growth.
Briefly, the cells were mechanically dissociated, suspended in Opti-MEM with 5% heat-inactivated fetal bovine serum, 0.2% N2 supplement (GIBCO) and 1% antibiotic solution (penicillin G 10 4units/ml, streptomycin 10 mg/ml and amphotericin B 25 μg/ml) and plated in flat bottom 96 well plates.
On the day before transfection, cells were plated in 6 cm diameter dishes at 5 × 10 5cells/dish or in 10 cm diameter dishes at 2 × 10 6cells/dish.
RPE cells were then removed under a dissecting microscope, dissociated by trituration using a Pasteur pipette, and then plated on poly-L-lysine (25 mg/liter in water) coated 35-cm 2culture dishes.
For example, 1.4 mM H 2 O 2 in a culture plated at a density of 40,000 cells/well should result in a viability close to twice of that generated in 20,000 cells/well and four times of that in 10,000 cells/well.