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Example sentences for: pjm

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pJM110 was constructed by cloning a Bam HI- and Nco I-cleaved PCR product of pcrG into pACT2.
Plasmids used in this study are described in Table 3. All plasmids expressing truncations of LcrG (pJM1, pJM2, pJM3, pJM4, pJM5, pJM23, pJM52, and pJM53) were constructed by cloning Bam HI- and Nco I-cleaved PCR products into pACT2.
Plasmid pJM127 was constructed by cloning an Eco RI-cleaved PCR p6roduct of pcrG into Eco RI- and Sma I-cleaved pBAD18.
pACTG was used as the template for all plasmids listed in Table 1and Table 2. pAraG18K was used as the template for pJM99, pJM131, pJM143, and pJM144.
pJM127 was digested with Eco RI and Xba I to release a pcrG fragment that was subsequently subcloned into EcoRI- and XbaI-cleaved pBAD18-Kan to create pJM132.